Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico

Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico

Tenderness, an important characteristic for marketing beef, has been associated with genetic factors, such as single nucleotide polymorphisms (SNPs). The aim of this work was to analyze the genotypic and allelic distribution of G530A, C357G, G1795A and G1181A SNPs to establish an association with me...

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Autors principals: Sanchez Mendoza, Ana Elvia, Fonseca Coronado, Salvador, Soto Zárate, Carlos Ignacio, López Hernández, Luis Humberto, Meléndez Pérez, Rosalía, Montiel Sosa, José Francisco
Format: Online
Idioma:spa
Publicat: Universidad de Sonora 2021
Accés en línia:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1098
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spelling biotecnia-article-10982022-05-31T17:19:20Z Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico Evaluación del efecto de los SNPs G530A (calpaína), C357G (calpastatina), G1795A (miopaladina) y G1181A (PPARGC1A) sobre la terneza de carne bovina de una región del Estado de Veracruz, México Sanchez Mendoza, Ana Elvia Fonseca Coronado, Salvador Soto Zárate, Carlos Ignacio López Hernández, Luis Humberto Meléndez Pérez, Rosalía Montiel Sosa, José Francisco SNPs PCR-RFLP cattle meat quality tenderness SNPs PCR-RFLP bovino calidad de la carne terneza Tenderness, an important characteristic for marketing beef, has been associated with genetic factors, such as single nucleotide polymorphisms (SNPs). The aim of this work was to analyze the genotypic and allelic distribution of G530A, C357G, G1795A and G1181A SNPs to establish an association with meat tenderness. Ninety samples of longissimus dorsi muscle were obtained. Tenderness was determined as a Warner-Bratzler shear force. SNPs genotyping was performed by PCR-RFLP. Using a generalized linear model (GLM), we evaluated the association between SNPs and tenderness. The effect of the alleles was determined by allelic substitution analysis. It was determined that the genetic group influenced tenderness (p <0.05). For C530A and G1181A SNPs, a lower frequency was obtained for the AA genotypes, while for C357G and G1795A SNPs higher frequencies were observed for the ancestral CC and GG genotypes. We found no genotypic or allelic association between SNPs and tenderness. This shows the importance of carrying out regional análisis of association between SNPs and quality characteristics to introduce them favorably through marker-assisted selection programs. La terneza es una característica importante para la comercialización de carne de bovino; el grado de terneza está asociado a factores genéticos como los polimorfismos de nucleótido único (SNPs). El objetivo de este trabajo fue evaluar los SNPs G530A, C357G, G1795A y G1181A como predictores de terneza. Se obtuvieron 90 muestras de musculo longissimus dorsi. La terneza se determinó como fuerza de corte de Warner-Bratzler. La genotipificación de los SNPs se realizó por PCR-RFLP. Se realizó una prueba de asociación entre los SNPs y la terneza mediante un modelo de efectos mixtos. Se determinó que el grupo genético influyó sobre la terneza (P < 0.05).  Para el SNP C530A se detectó baja frecuencia del genotipo AA (0.011), de forma similar para el SNP G1181A se obtuvo baja frecuencia para el genotipo AA (0.033), para los SNPs  C357G y G1795A se obtuvieron mayores frecuencias para los genotipos ancestrales CC (0.711) y GG (0.20), respectivamente. Los resultados establecieron que no existe influencia de estos SNPs (P > 0.05) sobre la terneza. Esto indica la necesidad de llevar a cabo un análisis de validación de los SNPs de interés, para poder introducirlos de manera favorable mediante programas de selección asistida por marcadores. Universidad de Sonora 2021-01-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1098 10.18633/biotecnia.v23i1.1098 Biotecnia; Vol. 23 No. 1 (2021): Enero-Abril; 13-20 Biotecnia; Vol. 23 Núm. 1 (2021): Enero-Abril; 13-20 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1098/480 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1098/523 Derechos de autor 2021 Biotecnia https://creativecommons.org/licenses/by-nc-sa/4.0
institution Biotecnia
collection OJS
language spa
format Online
author Sanchez Mendoza, Ana Elvia
Fonseca Coronado, Salvador
Soto Zárate, Carlos Ignacio
López Hernández, Luis Humberto
Meléndez Pérez, Rosalía
Montiel Sosa, José Francisco
spellingShingle Sanchez Mendoza, Ana Elvia
Fonseca Coronado, Salvador
Soto Zárate, Carlos Ignacio
López Hernández, Luis Humberto
Meléndez Pérez, Rosalía
Montiel Sosa, José Francisco
Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
author_facet Sanchez Mendoza, Ana Elvia
Fonseca Coronado, Salvador
Soto Zárate, Carlos Ignacio
López Hernández, Luis Humberto
Meléndez Pérez, Rosalía
Montiel Sosa, José Francisco
author_sort Sanchez Mendoza, Ana Elvia
title Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
title_short Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
title_full Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
title_fullStr Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
title_full_unstemmed Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico
title_sort evaluation of the effect of g530a (calpain), c357g (calpastatin), g1795a (myopaladin) and g1181a (ppargc1a) snps, on beef tenderness of a region of veracruz state, mexico
description Tenderness, an important characteristic for marketing beef, has been associated with genetic factors, such as single nucleotide polymorphisms (SNPs). The aim of this work was to analyze the genotypic and allelic distribution of G530A, C357G, G1795A and G1181A SNPs to establish an association with meat tenderness. Ninety samples of longissimus dorsi muscle were obtained. Tenderness was determined as a Warner-Bratzler shear force. SNPs genotyping was performed by PCR-RFLP. Using a generalized linear model (GLM), we evaluated the association between SNPs and tenderness. The effect of the alleles was determined by allelic substitution analysis. It was determined that the genetic group influenced tenderness (p <0.05). For C530A and G1181A SNPs, a lower frequency was obtained for the AA genotypes, while for C357G and G1795A SNPs higher frequencies were observed for the ancestral CC and GG genotypes. We found no genotypic or allelic association between SNPs and tenderness. This shows the importance of carrying out regional análisis of association between SNPs and quality characteristics to introduce them favorably through marker-assisted selection programs.
publisher Universidad de Sonora
publishDate 2021
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1098
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