Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis

Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis

Mycobacterium smegmatis (Ms), with its short generation time and low biosafety requirements, serves as an appropriate model to study Mycobacteria in general and is useful for assays of anti-tuberculosis agents. Recently, ceramic materials have been used in biomedical applications as a substrate. The...

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मुख्य लेखकों: Garibay Escobar, Adriana, Brown Bojórquez, Francisco, Vazquez Paz, Flor Madalitza, Muñoz Palma, Iliana Celina I., Pérez Tello, Manuel
स्वरूप: Online
भाषा:eng
प्रकाशित: Universidad de Sonora 2020
ऑनलाइन पहुंच:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1197
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spelling biotecnia-article-11972022-05-31T17:20:08Z Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis Síntesis de soportes cerámicos basados en HA+In2TiO5 para crecimiento acelerado de Mycobacterium smegmatis Garibay Escobar, Adriana Brown Bojórquez, Francisco Vazquez Paz, Flor Madalitza Muñoz Palma, Iliana Celina I. Pérez Tello, Manuel Ceramic materials Mycobacterium Photocatalysis Hydroxyapatite Materiales cerámicos , In2TiO5 Mycobacteria Fotocatálisis Hidroxiapatita Mycobacterium smegmatis (Ms), with its short generation time and low biosafety requirements, serves as an appropriate model to study Mycobacteria in general and is useful for assays of anti-tuberculosis agents. Recently, ceramic materials have been used in biomedical applications as a substrate. The aim of this work was to evaluate the ability of growth by Ms over supports prepared with a ceramic mixture. The supports were synthetized from hydroxyapatite (HA) and In2TiO5. SEM, EDS and XRD were used for analyze its final chemical composition. The increase of the oxygen concentration it is attributed to photocatalytic effect promoted by the sunlight exposure of supports immersed on MDB 7H9. The ceramic mixture, was responsible of the oxygen increase and, consequently, of the increase of eight times on the minimal inhibitory concentration in rezasurine assays on microplate. The use of the ceramic support showed the Ms growth 24 h before respect to the control without support.   Los materiales cerámicos han sido recientemente utilizados como sustratos en aplicaciones biomédicas. El objetivo de este trabajo, fue el de sintetizar soportes prepa­rados a partir de hidroxiapatita (HA) y titanato de indio (In­2TiO5) para su aplicación en el crecimiento de Mycobacterium smegmatis (Ms). In2TiO5, es un óxido ternario con estructura cristalina abierta que ha sido ampliamente estudiado princi­palmente por su resistencia al ambiente y tiene aplicaciones como refractario y fotocatalizador. Por otra parte, debido a su corto tiempo de generación y bajos requerimentos de bioseguridad, Ms, se considera un modelo apropiado para el estudio de Mycobacteria en general, y es útil para ensayos con agentes anti-tuberculosis. Los soportes cerámicos, se caracterizaron estructuralmente mediante MEB, EDX y DRX de polvos. El medio de cultivo MDB 7H9 conteniendo a los soportes, incrementó su concentración de oxígeno, lo que se atribuyó a la fotocátalisis promovida por In2TiO5 tras la exposición a la luz solar. Así mismo, el uso de los soportes cerámicos incrementó ocho veces la concentración mínima inhibitoria en los ensayos de rezasurina en microplaca. Adi­cionalmente, se encontró crecimiento de Ms 24 horas antes con respecto al control sin soporte, lo cual mejora el tiempo de obtención del diagnóstico. Universidad de Sonora 2020-09-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1197 10.18633/biotecnia.v22i3.1197 Biotecnia; Vol. 22 No. 3 (2020): Septiembre-Diciembre; 125-130 Biotecnia; Vol. 22 Núm. 3 (2020): Septiembre-Diciembre; 125-130 1665-1456 1665-1456 eng https://biotecnia.unison.mx/index.php/biotecnia/article/view/1197/464 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1197/517 Derechos de autor 2020 Biotecnia
institution Biotecnia
collection OJS
language eng
format Online
author Garibay Escobar, Adriana
Brown Bojórquez, Francisco
Vazquez Paz, Flor Madalitza
Muñoz Palma, Iliana Celina I.
Pérez Tello, Manuel
spellingShingle Garibay Escobar, Adriana
Brown Bojórquez, Francisco
Vazquez Paz, Flor Madalitza
Muñoz Palma, Iliana Celina I.
Pérez Tello, Manuel
Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
author_facet Garibay Escobar, Adriana
Brown Bojórquez, Francisco
Vazquez Paz, Flor Madalitza
Muñoz Palma, Iliana Celina I.
Pérez Tello, Manuel
author_sort Garibay Escobar, Adriana
title Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
title_short Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
title_full Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
title_fullStr Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
title_full_unstemmed Supports of ceramic mixture for acelerated growth of Mycobacterium smegmatis
title_sort supports of ceramic mixture for acelerated growth of mycobacterium smegmatis
description Mycobacterium smegmatis (Ms), with its short generation time and low biosafety requirements, serves as an appropriate model to study Mycobacteria in general and is useful for assays of anti-tuberculosis agents. Recently, ceramic materials have been used in biomedical applications as a substrate. The aim of this work was to evaluate the ability of growth by Ms over supports prepared with a ceramic mixture. The supports were synthetized from hydroxyapatite (HA) and In2TiO5. SEM, EDS and XRD were used for analyze its final chemical composition. The increase of the oxygen concentration it is attributed to photocatalytic effect promoted by the sunlight exposure of supports immersed on MDB 7H9. The ceramic mixture, was responsible of the oxygen increase and, consequently, of the increase of eight times on the minimal inhibitory concentration in rezasurine assays on microplate. The use of the ceramic support showed the Ms growth 24 h before respect to the control without support.  
publisher Universidad de Sonora
publishDate 2020
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1197
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