Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth)
Plants, being sessile organisms, suffer from a range of biotic and abiotic stress. In this investigation, oregano plants were grown in vitro under saline stress conditions (NaCl 25 mM), combined with different types of lights: white light (CTL), ultraviolet- C (UV-C) and broad-spectrum light (AE). M...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Online |
| Lenguaje: | spa |
| Publicado: |
Universidad de Sonora
2020
|
| Acceso en línea: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1223 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
biotecnia-article-1223 |
|---|---|
| record_format |
ojs |
| spelling |
biotecnia-article-12232022-05-31T17:20:08Z Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) Efecto del estrés salino sobre la morfología y fitoquímica de orégano mexicano (Lippia graveolens Kunth) cultivado in vitro Aarland, Rayn Castellanos-Hernandez, Osvaldo Rodriguez-Sahagun, Araceli Acevedo-Hernandez, Gustavo Lippia graveolens stress total phenols antioxidant capacity Lippia graveolens estrés fenoles totales capacidad antioxidante Plants, being sessile organisms, suffer from a range of biotic and abiotic stress. In this investigation, oregano plants were grown in vitro under saline stress conditions (NaCl 25 mM), combined with different types of lights: white light (CTL), ultraviolet- C (UV-C) and broad-spectrum light (AE). Morphological changes were evaluated in the treated plants, as well as phytochemical parameters (total phenolic compounds, total flavonoids and antioxidant capacity). NaCl/CTL and AE conditions showed the highest numbers of activated buds. UV-C light showed the lowest number of buds and plant height, however, no effect from salinity was observed. Also, UV-C light caused the lowest amount of leaves and adventitious roots, which do not seem to be influenced by salinity. The stress condition that caused the highest amount of phenols was UV-C light, while the combination of NaCl/ UV-C showed the highest number of total flavonoids. As for the antioxidant capacity, NaCl/AE showed the highest capacity using both the DPPH and ABTS method. No correlation between phenols and antioxidant capacity was observed. Las plantas, al ser organismos sésiles sufren diferentes tipos de estreses bióticos y abióticos. En este trabajo se cultivaron in vitro plantas de orégano, bajo condiciones de estrés salino (NaCl 25 mM), en diferentes fuentes de luz: blanca (CTL), ultravioleta tipo C (UV-C) y de amplio espectro (AE). Se evaluaron cambios morfológicos en las plantas tratadas, así como parámetros fitoquímicos (fenoles y flavonoides totales y la capacidad antioxidante). Los tratamientos NaCl/CTL y AE mostraron el mayor número de yemas activadas. La luz UV-C mostró la menor cantidad de yemas y de altura de planta, sin embargo, no se observó efecto de la salinidad. La luz UV-C causó el menor número de hojas, así como raíces adventicias. No parece haber injerencia de la condición salinidad en estos resultados. La condición que provocó el mayor contenido de fenoles fue la luz UV-C y la que provocó el mayor contenido de flavonoides fue la combinación NaCl/UV-C. La combinación NaCl/AE causó la mayor capacidad antioxidante con el método DPPH y ABTS. No se observó una correlación entre el contenido de fenoles y la capacidad antioxidante. Universidad de Sonora 2020-09-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1223 10.18633/biotecnia.v22i3.1223 Biotecnia; Vol. 22 No. 3 (2020): Septiembre-Diciembre; 131-137 Biotecnia; Vol. 22 Núm. 3 (2020): Septiembre-Diciembre; 131-137 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1223/465 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1223/518 Derechos de autor 2020 Biotecnia |
| institution |
Biotecnia |
| collection |
OJS |
| language |
spa |
| format |
Online |
| author |
Aarland, Rayn Castellanos-Hernandez, Osvaldo Rodriguez-Sahagun, Araceli Acevedo-Hernandez, Gustavo |
| spellingShingle |
Aarland, Rayn Castellanos-Hernandez, Osvaldo Rodriguez-Sahagun, Araceli Acevedo-Hernandez, Gustavo Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| author_facet |
Aarland, Rayn Castellanos-Hernandez, Osvaldo Rodriguez-Sahagun, Araceli Acevedo-Hernandez, Gustavo |
| author_sort |
Aarland, Rayn |
| title |
Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| title_short |
Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| title_full |
Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| title_fullStr |
Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| title_full_unstemmed |
Effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (Lippia graveolens Kunth) |
| title_sort |
effect of saline stress on the morphology and phytochemistry of in vitro grown mexican oregano (lippia graveolens kunth) |
| description |
Plants, being sessile organisms, suffer from a range of biotic and abiotic stress. In this investigation, oregano plants were grown in vitro under saline stress conditions (NaCl 25 mM), combined with different types of lights: white light (CTL), ultraviolet- C (UV-C) and broad-spectrum light (AE). Morphological changes were evaluated in the treated plants, as well as phytochemical parameters (total phenolic compounds, total flavonoids and antioxidant capacity). NaCl/CTL and AE conditions showed the highest numbers of activated buds. UV-C light showed the lowest number of buds and plant height, however, no effect from salinity was observed. Also, UV-C light caused the lowest amount of leaves and adventitious roots, which do not seem to be influenced by salinity. The stress condition that caused the highest amount of phenols was UV-C light, while the combination of NaCl/ UV-C showed the highest number of total flavonoids. As for the antioxidant capacity, NaCl/AE showed the highest capacity using both the DPPH and ABTS method. No correlation between phenols and antioxidant capacity was observed. |
| publisher |
Universidad de Sonora |
| publishDate |
2020 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1223 |
| work_keys_str_mv |
AT aarlandrayn effectofsalinestressonthemorphologyandphytochemistryofinvitrogrownmexicanoreganolippiagraveolenskunth AT castellanoshernandezosvaldo effectofsalinestressonthemorphologyandphytochemistryofinvitrogrownmexicanoreganolippiagraveolenskunth AT rodriguezsahagunaraceli effectofsalinestressonthemorphologyandphytochemistryofinvitrogrownmexicanoreganolippiagraveolenskunth AT acevedohernandezgustavo effectofsalinestressonthemorphologyandphytochemistryofinvitrogrownmexicanoreganolippiagraveolenskunth AT aarlandrayn efectodelestressalinosobrelamorfologiayfitoquimicadeoreganomexicanolippiagraveolenskunthcultivadoinvitro AT castellanoshernandezosvaldo efectodelestressalinosobrelamorfologiayfitoquimicadeoreganomexicanolippiagraveolenskunthcultivadoinvitro AT rodriguezsahagunaraceli efectodelestressalinosobrelamorfologiayfitoquimicadeoreganomexicanolippiagraveolenskunthcultivadoinvitro AT acevedohernandezgustavo efectodelestressalinosobrelamorfologiayfitoquimicadeoreganomexicanolippiagraveolenskunthcultivadoinvitro |
| _version_ |
1756584813469368320 |
