Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata

Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata

The objective of this work was to evaluate the effect of the Chrysactinia mexicana A. Gray essential oil, on C. glabrata clinical isolates. The essential oil was obtained by steam entrainment of flowers, leaves and secondary branches of C. mexicana. Gas chromatography and mass spectrometry were perf...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Gallegos-García, Verónica, Medina-de la Cruz, Omar, Leal-Morales, Carlos Alberto, Meza-Menchaca, Thuluz, Guillen, Larissa, Juárez-Flores, Bertha Irene
Formato: Online
Lenguaje:spa
Publicado: Universidad de Sonora 2021
Acceso en línea:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1265
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id biotecnia-article-1265
record_format ojs
spelling biotecnia-article-12652022-05-31T17:19:20Z Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata Efecto del aceite esencial de Chrysactinia mexicana A. Gray sobre aislados clínicos de Candida glabrata Gallegos-García, Verónica Medina-de la Cruz, Omar Leal-Morales, Carlos Alberto Meza-Menchaca, Thuluz Guillen, Larissa Juárez-Flores, Bertha Irene S. cerevisiae Fungicide Piperitone Asteraceae Microdilution S. cerevisiae Fungicida Piperitona Asteraceae Microdilución The objective of this work was to evaluate the effect of the Chrysactinia mexicana A. Gray essential oil, on C. glabrata clinical isolates. The essential oil was obtained by steam entrainment of flowers, leaves and secondary branches of C. mexicana. Gas chromatography and mass spectrometry were performed to determine the oil composition. Logarithmic and stationary phase microdilution assays were performed with Saccharomyces cerevisiae and clinical isolates of C. gla­brata to determine the Minimum Inhibitory Concentration (MIC) of the oil. The cultures were incubated in liquid YPD medium, for 7 h in fresh medium (logarithmic phase) or 3 h in recovering medium (stationary phase), with the oil and microdilution tests were performed. The three main oil compounds identified were piperitone (29.57%), eucalyptol (26.86%) and α-terpineol (14.65%). The MICs obtained were, a) stationary phase: S. cerevisiae (BY4741) 2.78 mg/mL and C. glabrata (CBS138), (NY66) and (AN400) 6.50 mg/mL and (NY62) 9.29 mg/mL; b) logarithmic phase: S. cerevisiae (BY4741) 3.71 mg/mL and C. glabrata (CBS138) 4.64 mg/mL, (NY66) 6.5 mg/mL, (AN400) 8.36 mg/mL, and (NY62) 18.5 mg/ mL. This work constitutes the first report of the fungicidal effect of the C. mexicana essential oil on human pathogenic fungi such as C. glabrata. El objetivo del trabajo fue evaluar el efecto del aceite esencial Chrysactinia mexicana A. Gray en aislados clínicos de C. glabrata. Se obtuvo aceite esencial por arrastre de vapor de las flores, hojas y ramas secundarias de C. mexicana. Se realizó cromatografía de gases y espectrometría de masas para conocer la composición del aceite. Se hicieron ensayos de microdilución en fase logarítmica y fase estacionaria con Saccharomyces cerevisiae y aislados clínicos de C. glabrata para determinar la Concentración Mínima Inhibitoria (CMI) en presencia del aceite esencial. Los principales compuestos del aceite esencial fueron la piperitona (29.57 %), eucaliptol (26.86 %), a-terpineol (14.65 %), delta-3-careno (12.37 %) y linalool (3.56 %). Se determinaron las CMI del aceite esencial, para las cinco cepas utilizadas en las dos fases de crecimiento, siendo menor la CMI para S. cerevisiae que para los diferentes aislados clínicos de C. glabrata. El aceite esencial de C. mexicana tiene efecto fungicida en S. cerevisiae y los aislados clínicos de C. glabrata. Universidad de Sonora 2021-01-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1265 10.18633/biotecnia.v23i1.1265 Biotecnia; Vol. 23 No. 1 (2021): Enero-Abril; 28-35 Biotecnia; Vol. 23 Núm. 1 (2021): Enero-Abril; 28-35 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1265/483 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1265/525 Derechos de autor 2021 Biotecnia
institution Biotecnia
collection OJS
language spa
format Online
author Gallegos-García, Verónica
Medina-de la Cruz, Omar
Leal-Morales, Carlos Alberto
Meza-Menchaca, Thuluz
Guillen, Larissa
Juárez-Flores, Bertha Irene
spellingShingle Gallegos-García, Verónica
Medina-de la Cruz, Omar
Leal-Morales, Carlos Alberto
Meza-Menchaca, Thuluz
Guillen, Larissa
Juárez-Flores, Bertha Irene
Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
author_facet Gallegos-García, Verónica
Medina-de la Cruz, Omar
Leal-Morales, Carlos Alberto
Meza-Menchaca, Thuluz
Guillen, Larissa
Juárez-Flores, Bertha Irene
author_sort Gallegos-García, Verónica
title Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
title_short Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
title_full Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
title_fullStr Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
title_full_unstemmed Effect of the essential oil of Chrysactinia mexicana A. Gray on clinical isolates of Candida glabrata
title_sort effect of the essential oil of chrysactinia mexicana a. gray on clinical isolates of candida glabrata
description The objective of this work was to evaluate the effect of the Chrysactinia mexicana A. Gray essential oil, on C. glabrata clinical isolates. The essential oil was obtained by steam entrainment of flowers, leaves and secondary branches of C. mexicana. Gas chromatography and mass spectrometry were performed to determine the oil composition. Logarithmic and stationary phase microdilution assays were performed with Saccharomyces cerevisiae and clinical isolates of C. gla­brata to determine the Minimum Inhibitory Concentration (MIC) of the oil. The cultures were incubated in liquid YPD medium, for 7 h in fresh medium (logarithmic phase) or 3 h in recovering medium (stationary phase), with the oil and microdilution tests were performed. The three main oil compounds identified were piperitone (29.57%), eucalyptol (26.86%) and α-terpineol (14.65%). The MICs obtained were, a) stationary phase: S. cerevisiae (BY4741) 2.78 mg/mL and C. glabrata (CBS138), (NY66) and (AN400) 6.50 mg/mL and (NY62) 9.29 mg/mL; b) logarithmic phase: S. cerevisiae (BY4741) 3.71 mg/mL and C. glabrata (CBS138) 4.64 mg/mL, (NY66) 6.5 mg/mL, (AN400) 8.36 mg/mL, and (NY62) 18.5 mg/ mL. This work constitutes the first report of the fungicidal effect of the C. mexicana essential oil on human pathogenic fungi such as C. glabrata.
publisher Universidad de Sonora
publishDate 2021
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1265
work_keys_str_mv AT gallegosgarciaveronica effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT medinadelacruzomar effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT lealmoralescarlosalberto effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT mezamenchacathuluz effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT guillenlarissa effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT juarezfloresberthairene effectoftheessentialoilofchrysactiniamexicanaagrayonclinicalisolatesofcandidaglabrata
AT gallegosgarciaveronica efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
AT medinadelacruzomar efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
AT lealmoralescarlosalberto efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
AT mezamenchacathuluz efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
AT guillenlarissa efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
AT juarezfloresberthairene efectodelaceiteesencialdechrysactiniamexicanaagraysobreaisladosclinicosdecandidaglabrata
_version_ 1756584817681498112

Publicaciones similares