Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney

Thraustochytrids are characterized by producing omega 3 and 6 type microbial oils. VAL-B1 and EMA-T5 are strains of the same genus and take advantage of pure sources of carbon and residual to produce polyunsaturated fatty acids. Pure (GP) and crude (GC) glycerol were used to compare biomass producti...

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Egile Nagusiak:	Silva Rodríguez, David, Beltrán-Guilarte, Yayne, Angulo-Mercado, Eduardo R., Quilodrán-Toloza, Benita H.
Formatua:	Online
Hizkuntza:	spa
Argitaratua:	Universidad de Sonora 2021
Sarrera elektronikoa:	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1405
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record_format	ojs
spelling	biotecnia-article-14052022-05-31T17:38:57Z Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney Estudio comparativo de la producción de biomasa y omega 3 y 6 en Thraustochytrium kinney Silva Rodríguez, David Beltrán-Guilarte, Yayne Angulo-Mercado, Eduardo R. Quilodrán-Toloza, Benita H. Polyunsaturated fatty acids, crude glycerol, microbial oils, docosahexaenoic acid. Ácidos grasos poliinsaturados, glicerol crudo, aceites microbianos, ácido docosahexaenoico (DHA). Thraustochytrids are characterized by producing omega 3 and 6 type microbial oils. VAL-B1 and EMA-T5 are strains of the same genus and take advantage of pure sources of carbon and residual to produce polyunsaturated fatty acids. Pure (GP) and crude (GC) glycerol were used to compare biomass production and omega 3 and 6 yield and production, using 3 different substrate concentrations. Using 100 mL of culture at 25 ° C and 180 rpm, the strains were grown for 7 days, then the biomass was centrifuged and the fatty acid profile was quantified by gas chromatography. The results show that EMA-T5 generates a greater amount of biomass at day 5 using 20 g/L of GC with 6.984±0.66 g/L. Regarding the yield and production of omega 3 and 6, VAL-B1 obtained the best results with 91.80±8.78 mg/g and 1167.71±70.88 mg/L, being the most abundant omega 3, especially DHA which presented the highest abundance with 52.72±4.94 % total fatty acids (TFA). It is concluded that EMA-T5 generates higher biomass production using GC and VAL-B1 presents the best performance and omega production results using GP, which indicates that both strains make good use of both the pure and raw sources of glycerol. Thraustochytridos se caracterizan por producir aceites microbianos del tipo omega 3 y 6. VAL-B1 y EMA-T5 son cepas del mismo género y aprovechan fuentes puras de carbono y residual para producir ácidos grasos poliinsaturados. Se utilizó glicerol puro (GP) y crudo (GC) para comparar la producción de biomasa y el rendimiento y producción de omega 3 y 6, usando 3 concentraciones diferentes de sustrato. Empleando 100 mL de cultivo a 25°C y 180 rpm, se  crecieron las cepas por 7 días, luego se centrifugaron y cuantifico la biomasa y por cromatografía gaseosa el perfil de ácidos grasos. Los resultados muestran que EMA-T5 genera mayor cantidad de biomasa al 5 día usando 20 g/L de GC con 6,984±0,66 g/L. Respecto al rendimiento y producción de omega 3 y 6, VAL-B1 obtuvo los mejores resultados con 91,80±8,78 mg/g y 1167,71±70,88 mg/L, siendo el omega 3 más abundante, en especial DHA con 52,72±4,94 %  de ácidos grasos totales (AGT). Se concluye, que EMA-T5 genera mayor producción de biomasa usando GC y VAL-B1 presenta los mejores resultados de rendimiento y producción de omega empleando GP, lo cual indica que ambas cepas, aprovechan de buena forma tanto la fuente pura como cruda del glicerol. Universidad de Sonora 2021-06-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1405 10.18633/biotecnia.v23i2.1405 Biotecnia; Vol. 23 No. 2 (2021): Mayo-Agosto Biotecnia; Vol. 23 Núm. 2 (2021): Mayo-Agosto 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1405/551 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1405/613 Derechos de autor 2021 https://creativecommons.org/licenses/by-nc-sa/4.0
institution	Biotecnia
collection	OJS
language	spa
format	Online
author	Silva Rodríguez, David Beltrán-Guilarte, Yayne Angulo-Mercado, Eduardo R. Quilodrán-Toloza, Benita H.
spellingShingle	Silva Rodríguez, David Beltrán-Guilarte, Yayne Angulo-Mercado, Eduardo R. Quilodrán-Toloza, Benita H. Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
author_facet	Silva Rodríguez, David Beltrán-Guilarte, Yayne Angulo-Mercado, Eduardo R. Quilodrán-Toloza, Benita H.
author_sort	Silva Rodríguez, David
title	Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
title_short	Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
title_full	Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
title_fullStr	Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
title_full_unstemmed	Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
title_sort	comparative study of the production of biomass and omega 3 and 6 in thraustochytrium kinney
description	Thraustochytrids are characterized by producing omega 3 and 6 type microbial oils. VAL-B1 and EMA-T5 are strains of the same genus and take advantage of pure sources of carbon and residual to produce polyunsaturated fatty acids. Pure (GP) and crude (GC) glycerol were used to compare biomass production and omega 3 and 6 yield and production, using 3 different substrate concentrations. Using 100 mL of culture at 25 ° C and 180 rpm, the strains were grown for 7 days, then the biomass was centrifuged and the fatty acid profile was quantified by gas chromatography. The results show that EMA-T5 generates a greater amount of biomass at day 5 using 20 g/L of GC with 6.984±0.66 g/L. Regarding the yield and production of omega 3 and 6, VAL-B1 obtained the best results with 91.80±8.78 mg/g and 1167.71±70.88 mg/L, being the most abundant omega 3, especially DHA which presented the highest abundance with 52.72±4.94 % total fatty acids (TFA). It is concluded that EMA-T5 generates higher biomass production using GC and VAL-B1 presents the best performance and omega production results using GP, which indicates that both strains make good use of both the pure and raw sources of glycerol.
publisher	Universidad de Sonora
publishDate	2021
url	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1405
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Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney

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