Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos
Mammals experience some degree of hypoxia during their lifetime. In response to hypoxic challenge, mammalian cells orchestrate specific responses at transcriptional and posttranslational level which lead to changes in the purine metabolites in order to cope with threatening conditions. The aim of th...
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| Formatua: | Online |
| Hizkuntza: | eng |
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Universidad de Sonora
2021
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| Sarrera elektronikoa: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1444 |
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biotecnia-article-1444 |
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Biotecnia |
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| language |
eng |
| format |
Online |
| author |
Rivera-Pérez, Crisalejandra Gaxiola-Robles, Ramón Olguín-Monroy, Norma Lugo-Lugo, Orlando López-Cruz, Roberto I. Zenteno-Savín, Tania |
| spellingShingle |
Rivera-Pérez, Crisalejandra Gaxiola-Robles, Ramón Olguín-Monroy, Norma Lugo-Lugo, Orlando López-Cruz, Roberto I. Zenteno-Savín, Tania Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| author_facet |
Rivera-Pérez, Crisalejandra Gaxiola-Robles, Ramón Olguín-Monroy, Norma Lugo-Lugo, Orlando López-Cruz, Roberto I. Zenteno-Savín, Tania |
| author_sort |
Rivera-Pérez, Crisalejandra |
| title |
Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| title_short |
Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| title_full |
Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| title_fullStr |
Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| title_full_unstemmed |
Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| title_sort |
efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos |
| description |
Mammals experience some degree of hypoxia during their lifetime. In response to hypoxic challenge, mammalian cells orchestrate specific responses at transcriptional and posttranslational level which lead to changes in the purine metabolites in order to cope with threatening conditions. The aim of this study was to evaluate the response of the enzymes involved in the purine metabolism of human muscle cells to hypoxic conditions. Muscle cells in culture were exposed to hypoxia and the enzymatic activity of inosine monophosphate dehydrogenase (IMPDH), xanthine oxidase (XO), purine nucleoside phosphorylase (PNP) and hypoxanthine guanine phosphoribosyl transferase (HGPRT) as well as their transcript expression were quantified under normoxic and hypoxic conditions. Purine metabolite (hypoxanthine (HX), xanthine (X), uric acid (UA), inosine monophosphate (IMP), inosine, nicotinamide adenine dinucleotide (NAD+), adenosine, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), guanosine diphosphate (GDP) and guanosine triphosphate (GTP)) concentrations were also quantified. Significant reduction of IMPDH activity and HX and IMP concentrations (p < 0.05) were observed after hypoxia, suggesting a decrease of de novo synthesis of purines. After hypoxia a global reduction of transcripts was observed, suggesting a reduction of the metabolic machinery of purine metabolism to new steady states that balance ATP demand and ATP supply pathways. |
| publisher |
Universidad de Sonora |
| publishDate |
2021 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1444 |
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| _version_ |
1756584826694008832 |
| spelling |
biotecnia-article-14442022-05-31T17:38:57Z Efecto de la hipoxia en el metabolismo de purinas en células de músculo esquelético de humanos Effect of hypoxia on purine metabolism in human skeletal muscle cells Rivera-Pérez, Crisalejandra Gaxiola-Robles, Ramón Olguín-Monroy, Norma Lugo-Lugo, Orlando López-Cruz, Roberto I. Zenteno-Savín, Tania hypoxia cell culture purines salvage pathway English Hipoxia cultivo celular purinas vía de rescate Mammals experience some degree of hypoxia during their lifetime. In response to hypoxic challenge, mammalian cells orchestrate specific responses at transcriptional and posttranslational level which lead to changes in the purine metabolites in order to cope with threatening conditions. The aim of this study was to evaluate the response of the enzymes involved in the purine metabolism of human muscle cells to hypoxic conditions. Muscle cells in culture were exposed to hypoxia and the enzymatic activity of inosine monophosphate dehydrogenase (IMPDH), xanthine oxidase (XO), purine nucleoside phosphorylase (PNP) and hypoxanthine guanine phosphoribosyl transferase (HGPRT) as well as their transcript expression were quantified under normoxic and hypoxic conditions. Purine metabolite (hypoxanthine (HX), xanthine (X), uric acid (UA), inosine monophosphate (IMP), inosine, nicotinamide adenine dinucleotide (NAD+), adenosine, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), guanosine diphosphate (GDP) and guanosine triphosphate (GTP)) concentrations were also quantified. Significant reduction of IMPDH activity and HX and IMP concentrations (p < 0.05) were observed after hypoxia, suggesting a decrease of de novo synthesis of purines. After hypoxia a global reduction of transcripts was observed, suggesting a reduction of the metabolic machinery of purine metabolism to new steady states that balance ATP demand and ATP supply pathways. Los mamíferos experimentan cierto grado de hipoxia durante su vida. Como respuesta al reto de hipoxia, las células de mamíferos orquestan respuestas específicas a nivel transcripcional y postraduccional que conducen a cambios en los metabolitos de purinas para hacer frente a las condiciones amenazantes. El objetivo de este estudio fue evaluar la respuesta de las enzimas involucradas en el metabolismo de las purinas de células musculares humanas a condiciones hipóxicas. Las células musculares en cultivo se expusieron a hipoxia y la actividad enzimática de la inosina monofosfato deshidrogenasa (IMPDH), la xantina oxidasa (XO), la purina nucleósido fosforilasa (PNP) y la hipoxantina guanina fosforribosil transferasa (HGPRT), así como su expresión de transcripción, se cuantificaron bajo condiciones de normoxia e hipoxia. Los metabolitos de purina (hipoxantina (HX), xantina (X), ácido úrico (UA), monofosfato de inosina (IMP), inosina, dinucleótido de nicotinamida y adenina (NAD+), adenosina, monofosfato de adenosina (AMP), difosfato de adenosina (ADP), trifosfato de adenosina (ATP), difosfato de guanosina (GDP) y trifosfato de guanosina (GTP)) también se cuantificaron. Se observó una reducción significativa de la actividad de IMPDH y de las concentraciones de HX e IMP (p < 0.05) después de la hipoxia, lo que sugiere una disminución de la síntesis de novo de purinas. Después de la hipoxia, se observó una reducción global de la expresión transcripcional, lo que sugiere una reducción de la maquinaria metabólica del metabolismo de las purinas a nuevos estados estacionarios que equilibran la demanda de ATP y las vías de suministro de ATP. Universidad de Sonora 2021-06-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1444 10.18633/biotecnia.v23i2.1444 Biotecnia; Vol. 23 No. 2 (2021): Mayo-Agosto Biotecnia; Vol. 23 Núm. 2 (2021): Mayo-Agosto 1665-1456 1665-1456 eng https://biotecnia.unison.mx/index.php/biotecnia/article/view/1444/556 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1444/622 Derechos de autor 2021 https://creativecommons.org/licenses/by-nc-sa/4.0 |
