Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper
The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growthpromoting bacteria. This research aimed to isolate growthpromoting bacteria, id...
Zapisane w:
| Główni autorzy: | , , , , , , |
|---|---|
| Format: | Online |
| Język: | spa |
| Wydane: |
Universidad de Sonora
2021
|
| Dostęp online: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480 |
| Etykiety: |
Dodaj etykietę
Nie ma etykietki, Dołącz pierwszą etykiete!
|
| id |
biotecnia-article-1480 |
|---|---|
| record_format |
ojs |
| spelling |
biotecnia-article-14802022-05-31T17:39:51Z Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper Identificación molecular y evaluación de bacterias en el desarrollo vegetativo y producción de chile habanero Adame García, Jacel Murillo Cuevas, Félix David Velázquez Mendoza, Víctor López Vázquez , Mario Antonio Vázquez, Efraín Cabrera Mireles, Héctor Villegas Narváez, Jazmín Bacillus biofertilizers vegetables germination Bacillus biofertilizantes hortalizas germinación The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growthpromoting bacteria. This research aimed to isolate growthpromoting bacteria, identify them molecularly, and evaluate their effects on the vegetative development and production of habanero pepper. Five bacterial strains were isolated and identified from samples from different land uses; the agroforestry system recorded on average the most significant amount (3.5x105 CFU g-1) of bacterial strains. Analysis of the 16S ribosomal DNA gene indicated that the bacteria were associated with species belonging to the Bacillus genus; however, the phylogenetic study shows that the identification at a species level is not possible; these strains stimulated better the seedlings growth (6.86, 6.70 and 6.76 cm, respectively). In the macro tunnel greenhouse evaluation, the EAV2 strain best stimulated the development of plants (38.63 cm) and produced better fruit weight (6.45 g). La producción de chile habanero se realiza con fertilización química, lo cual contamina el suelo y los mantos acuíferos. Esto ha motivado la búsqueda de alternativas sostenibles como las bacterias promotoras de crecimiento. El objetivo de esta investigación fue aislar bacterias promotoras del crecimiento, identificarlas molecularmente y evaluar sus efectos en el desarrollo vegetativo y producción de chile habanero. Se aislaron e identificaron cinco cepas bacterianas a partir de muestras de distintos usos de suelo; el sistema agroforestal registró en promedio la mayor cantidad (3.5x105 UFCg-1) de cepas bacterianas. El análisis del gen ADN ribosomal 16S indicó que las bacterias se asocian a diferentes especies del género Bacillus, sin embargo, el estudio filogenético demostró que no es posible aún identificarlas a nivel de especie; estas cepas estimularon el crecimiento de las plántulas (6.86, 6.70 y 6.76 cm, respectivamente). En la evaluación en macrotúnel, la cepa EAV2 fue la que estimuló el desarrollo de plantas (38.63 cm) y mejor peso de frutos (6.45 g). Universidad de Sonora 2021-10-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480 10.18633/biotecnia.v23i3.1480 Biotecnia; Vol. 23 No. 3 (2021): Septiembre-Diciembre Biotecnia; Vol. 23 Núm. 3 (2021): Septiembre-Diciembre 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480/581 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480/602 Derechos de autor 2021 https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Biotecnia |
| collection |
OJS |
| language |
spa |
| format |
Online |
| author |
Adame García, Jacel Murillo Cuevas, Félix David Velázquez Mendoza, Víctor López Vázquez , Mario Antonio Vázquez, Efraín Cabrera Mireles, Héctor Villegas Narváez, Jazmín |
| spellingShingle |
Adame García, Jacel Murillo Cuevas, Félix David Velázquez Mendoza, Víctor López Vázquez , Mario Antonio Vázquez, Efraín Cabrera Mireles, Héctor Villegas Narváez, Jazmín Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| author_facet |
Adame García, Jacel Murillo Cuevas, Félix David Velázquez Mendoza, Víctor López Vázquez , Mario Antonio Vázquez, Efraín Cabrera Mireles, Héctor Villegas Narváez, Jazmín |
| author_sort |
Adame García, Jacel |
| title |
Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| title_short |
Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| title_full |
Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| title_fullStr |
Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| title_full_unstemmed |
Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| title_sort |
molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper |
| description |
The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growthpromoting bacteria. This research aimed to isolate growthpromoting bacteria, identify them molecularly, and evaluate their effects on the vegetative development and production of habanero pepper. Five bacterial strains were isolated and identified from samples from different land uses; the agroforestry system recorded on average the most significant amount (3.5x105 CFU g-1) of bacterial strains. Analysis of the 16S ribosomal DNA gene indicated that the bacteria were associated with species belonging to the Bacillus genus; however, the phylogenetic study shows that the identification at a species level is not possible; these strains stimulated better the seedlings growth (6.86, 6.70 and 6.76 cm, respectively). In the macro tunnel greenhouse evaluation, the EAV2 strain best stimulated the development of plants (38.63 cm) and produced better fruit weight (6.45 g). |
| publisher |
Universidad de Sonora |
| publishDate |
2021 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480 |
| work_keys_str_mv |
AT adamegarciajacel molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT murillocuevasfelixdavid molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT velazquezmendozavictor molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT lopezvazquezmario molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT antoniovazquezefrain molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT cabreramireleshector molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT villegasnarvaezjazmin molecularidentificationandevaluationofbacteriainthevegetativedevelopmentandproductionofhabaneropepper AT adamegarciajacel identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT murillocuevasfelixdavid identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT velazquezmendozavictor identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT lopezvazquezmario identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT antoniovazquezefrain identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT cabreramireleshector identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero AT villegasnarvaezjazmin identificacionmolecularyevaluaciondebacteriaseneldesarrollovegetativoyproducciondechilehabanero |
| _version_ |
1756584828288892928 |
