Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry
The use of enzymes such as α-amylases, xylanases and glucosidases is a good alternative to obtain hihger efficiency in the products of the bakery industry, without leaving to mention the laccase that it has shown promising potential. Thus, the objective of this study was to obtain enzyme concentrate...
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Universidad de Sonora
2022
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| Acceso en línea: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1529 |
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biotecnia-article-15292022-05-31T17:40:22Z Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry Uso de cepas de Pycnoporus sanguineus para la producción de enzimas con potencial uso en la industria de la panificación Salinas Sánchez, Susana Daniela López-Sandin, Iosvany Hernández-Luna, Carlos E. Contreras-Cordero, Juan Francisco Méndez-Zamora, Gerardo Hernández-Martínez, Carlos A. Gutiérrez-Soto, Guadalupe basidiomycete carbohydrolases laccase native strain basidiomicetos carbohidrolasas lacasa cepas nativas The use of enzymes such as α-amylases, xylanases and glucosidases is a good alternative to obtain hihger efficiency in the products of the bakery industry, without leaving to mention the laccase that it has shown promising potential. Thus, the objective of this study was to obtain enzyme concentrates of xylanases, amylases, cellulases and laccases from native strains of Pycnoporus sanguineus. For which, the effect of the culture medium on the laccase production was evaluated together with the solid medium screening of xylanases, amylases and cellulases. Laccase and carbohydrolases units were determined from the enzyme concentrates obtained. The higher laccase titers occurred in the Tx2 medium. Regarding carbohydrolases, they were showed up at different levels of activity and combinations. The concentrate with higher titers of amylase and laccase was CH116, while LE90 had the greater xylanase activity and LE133 of cellulase. The CH116 strain was a producer of amylase, cellulase, xylanase and laccase, being the one with the higher potential in the bakery industry, while LE90 can be used in the paper industry due to its combination of xylanases and laccase. El uso de enzimas como α-amilasas, xilanasas y glucosidasas es una buena alternativa para obtener una mayor eficiencia en los productos de la industria panificadora, sin dejar de mencionar la lacasa que ha mostrado un potencial prometedor. Así, el objetivo de este estudio fue obtener concentrados enzimáticos de xilanasas, amilasas, celulasas y lacasas a partir de cepas nativas de Pycnoporus sanguineus. Para lo cual, se evaluó el efecto del medio de cultivo sobre la producción de lacasa juntamente con el escrutinio en medio sólido para la detección de xilanasas, amilasas y celulasas. A los concentrados enzimáticos obtenidos se les determinaron las unidades de lacasa y carbohidrolasas. Los títulos más altos de lacasa se presentaron en el medio Tx2. Con respecto a las carbohidrolasas, se detectaron en diferentes niveles de actividad y combinaciones. El concentrado con altos niveles de amilasa y lacasa fue CH116, mientras que LE90 tuvo la mayor actividad de xilanasa y LE133 de celulasa. La cepa CH116 fue productora de amilasa, celulasa, xilanasa y lacasa, siendo la de mayor potencial en la industria panificadora, mientras que LE90 puede ser utilizada en la industria papelera por su combinación de xilanasas y lacasa. Universidad de Sonora 2022-02-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1529 10.18633/biotecnia.v24i1.1529 Biotecnia; Vol. 24 No. 1 (2022): Enero-Abril; 62-68 Biotecnia; Vol. 24 Núm. 1 (2022): Enero-Abril; 62-68 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1529/633 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1529/670 Derechos de autor 2022 https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Biotecnia |
| collection |
OJS |
| language |
spa |
| format |
Online |
| author |
Salinas Sánchez, Susana Daniela López-Sandin, Iosvany Hernández-Luna, Carlos E. Contreras-Cordero, Juan Francisco Méndez-Zamora, Gerardo Hernández-Martínez, Carlos A. Gutiérrez-Soto, Guadalupe |
| spellingShingle |
Salinas Sánchez, Susana Daniela López-Sandin, Iosvany Hernández-Luna, Carlos E. Contreras-Cordero, Juan Francisco Méndez-Zamora, Gerardo Hernández-Martínez, Carlos A. Gutiérrez-Soto, Guadalupe Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| author_facet |
Salinas Sánchez, Susana Daniela López-Sandin, Iosvany Hernández-Luna, Carlos E. Contreras-Cordero, Juan Francisco Méndez-Zamora, Gerardo Hernández-Martínez, Carlos A. Gutiérrez-Soto, Guadalupe |
| author_sort |
Salinas Sánchez, Susana Daniela |
| title |
Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| title_short |
Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| title_full |
Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| title_fullStr |
Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| title_full_unstemmed |
Use of Pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| title_sort |
use of pycnoporus sanguineus strains to the production of enzymes with potential applications on the bread industry |
| description |
The use of enzymes such as α-amylases, xylanases and glucosidases is a good alternative to obtain hihger efficiency in the products of the bakery industry, without leaving to mention the laccase that it has shown promising potential. Thus, the objective of this study was to obtain enzyme concentrates of xylanases, amylases, cellulases and laccases from native strains of Pycnoporus sanguineus. For which, the effect of the culture medium on the laccase production was evaluated together with the solid medium screening of xylanases, amylases and cellulases. Laccase and carbohydrolases units were determined from the enzyme concentrates obtained. The higher laccase titers occurred in the Tx2 medium. Regarding carbohydrolases, they were showed up at different levels of activity and combinations. The concentrate with higher titers of amylase and laccase was CH116, while LE90 had the greater xylanase activity and LE133 of cellulase. The CH116 strain was a producer of amylase, cellulase, xylanase and laccase, being the one with the higher potential in the bakery industry, while LE90 can be used in the paper industry due to its combination of xylanases and laccase. |
| publisher |
Universidad de Sonora |
| publishDate |
2022 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1529 |
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