Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize
Quality protein maize (QPM) was created converting the soft opaque-2 endosperm into a vitreous phenotype, but the mechanisms involved in this modification are not completely understood. Recombinant inbred lines (RILs) derived from K0326Y QPM and W64Ao2 were used to identify quantitative trait loci (...
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Universidad de Sonora
2022
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| Acceso en línea: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1725 |
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Vega-Álvarez, Elthon Pineda-Hidalgo, Karen Virginia Salazar-Salas, Nancy Yareli Soto-López, Omar Alejandro Canizalez-Román, Vicente Adrián Garzón-Tiznado, José Antonio Gutiérrez-Dorado, Roberto Lopez-Valenzuela, Jose A. |
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Vega-Álvarez, Elthon Pineda-Hidalgo, Karen Virginia Salazar-Salas, Nancy Yareli Soto-López, Omar Alejandro Canizalez-Román, Vicente Adrián Garzón-Tiznado, José Antonio Gutiérrez-Dorado, Roberto Lopez-Valenzuela, Jose A. Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| author_facet |
Vega-Álvarez, Elthon Pineda-Hidalgo, Karen Virginia Salazar-Salas, Nancy Yareli Soto-López, Omar Alejandro Canizalez-Román, Vicente Adrián Garzón-Tiznado, José Antonio Gutiérrez-Dorado, Roberto Lopez-Valenzuela, Jose A. |
| author_sort |
Vega-Álvarez, Elthon |
| title |
Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| title_short |
Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| title_full |
Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| title_fullStr |
Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| title_full_unstemmed |
Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| title_sort |
genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize |
| description |
Quality protein maize (QPM) was created converting the soft opaque-2 endosperm into a vitreous phenotype, but the mechanisms involved in this modification are not completely understood. Recombinant inbred lines (RILs) derived from K0326Y QPM and W64Ao2 were used to identify quantitative trait loci (QTL) associated with starch physicochemical properties. RILs contrasting in vitreousness were also used to evaluate the expression of starch biosynthesis genes. Mapping identified 5-6 QTLs for each trait that explained 66 % of the phenotypic variation. Three QTLs on bins 4.05, 5.04, and 9.03 were found close to the starch biosynthesis genes Brittle-2 (Bt2), Amylose extender-1 (Ae1), and Waxy-1 (Wx1), respectively. The expression of Wx1 was three-fold greater in K0326Y QPM than W64Ao2 and eight-fold higher in vitreous than opaque RILs, which corresponded with the greater accumulation of granule bound starch synthase I (GBSSI) and the higher amylose content observed in the vitreous lines. The increased synthesis of amylose may result in starch granules with more amorphous regions that favor their compaction. These results suggest that endosperm modification in QPM is associated with the synthesis of starch containing more amylose during kernel development, which may facilitate the packing of the starch granules resulting in the vitreous phenotype. |
| publisher |
Universidad de Sonora |
| publishDate |
2022 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1725 |
| work_keys_str_mv |
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1756584837021433856 |
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biotecnia-article-17252022-10-28T15:06:34Z Genetic and molecular analysis of starch physicochemical properties and its relationship with endosperm modification in quality protein maize Análisis genético y molecular de propiedades fisicoquímicas del almidón y su asociación con la modificación del endospermo en maíz de calidad proteínica Vega-Álvarez, Elthon Pineda-Hidalgo, Karen Virginia Salazar-Salas, Nancy Yareli Soto-López, Omar Alejandro Canizalez-Román, Vicente Adrián Garzón-Tiznado, José Antonio Gutiérrez-Dorado, Roberto Lopez-Valenzuela, Jose A. Quality protein maize endosperm modification starch gene expression Maíz de calidad proteínica modificación del endospermo almidón expresión génica Quality protein maize (QPM) was created converting the soft opaque-2 endosperm into a vitreous phenotype, but the mechanisms involved in this modification are not completely understood. Recombinant inbred lines (RILs) derived from K0326Y QPM and W64Ao2 were used to identify quantitative trait loci (QTL) associated with starch physicochemical properties. RILs contrasting in vitreousness were also used to evaluate the expression of starch biosynthesis genes. Mapping identified 5-6 QTLs for each trait that explained 66 % of the phenotypic variation. Three QTLs on bins 4.05, 5.04, and 9.03 were found close to the starch biosynthesis genes Brittle-2 (Bt2), Amylose extender-1 (Ae1), and Waxy-1 (Wx1), respectively. The expression of Wx1 was three-fold greater in K0326Y QPM than W64Ao2 and eight-fold higher in vitreous than opaque RILs, which corresponded with the greater accumulation of granule bound starch synthase I (GBSSI) and the higher amylose content observed in the vitreous lines. The increased synthesis of amylose may result in starch granules with more amorphous regions that favor their compaction. These results suggest that endosperm modification in QPM is associated with the synthesis of starch containing more amylose during kernel development, which may facilitate the packing of the starch granules resulting in the vitreous phenotype. El maíz de calidad proteínica (QPM) fue creado convirtiendo el endospermo suave opaco-2 en un fenotipo vítreo, pero los mecanismos involucrados en esta modificación no se conocen por completo. Se utilizaron líneas recombinantes puras derivadas de las líneas K0326Y QPM y W64Ao2 para identificar loci de características cuantitativas (QTL) asociados con propiedades fisicoquímicas del almidón. También se usaron RILs contrastantes en vitrosidad para evaluar la expresión de genes de biosíntesis de almidón. El mapeo identificó 5 o 6 QTL para cada característica que explicaron en promedio el 66 % de la variación fenotípica. Tres de los QTLs en los bins 4.05, 5.04 y 9.03 se encontraron cerca de los genes de síntesis de almidón Brittle 2 (Bt2), Amylose extender 1 (Ae1), y Waxy 1 (Wx1), respectivamente. La expresión de Wx1 fue tres veces mayor en K0326Y QPM que en W64Ao2 y ocho veces mayor en líneas vítreas en comparación con las opacas, lo que correspondió con la mayor acumulación de la enzima almidón sintasa unida al gránulo I (GBSSI) y el mayor contenido de amilosa observado en las líneas vítreas. El incremento en la síntesis de amilosa podría resultar en gránulos de almidón con más regiones amorfas que favorecen su compactación. Estos resultados sugieren que la modificación del endospermo en QPM está asociada con la síntesis de almidón conteniendo más amilosa durante el desarrollo del grano, lo cual podría facilitar el empaquetamiento de los gránulos de almidón resultando en el fenotipo vítreo. Universidad de Sonora 2022-10-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf https://biotecnia.unison.mx/index.php/biotecnia/article/view/1725 10.18633/biotecnia.v24i3.1725 Biotecnia; Vol. 24 No. 3 (2022): Septiembre-Diciembre; 140 - 149 Biotecnia; Vol. 24 Núm. 3 (2022): Septiembre-Diciembre; 140 - 149 1665-1456 1665-1456 eng https://biotecnia.unison.mx/index.php/biotecnia/article/view/1725/720 Derechos de autor 2022 https://creativecommons.org/licenses/by-nc-sa/4.0 |
