Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms

Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms

Tagetes is a group of plants commonly known as cempasúchil or “flower of the dead”, which are mainly recognized for their use in the food industry, ornamental horticulture, therapeutic herbalism and culture. Tagetes coronopifolia and terniflora are two species plants of this genus, which are used in...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Castillo-Ortega, Laura Sofía, Mercado-Flores, Yuridia, Téllez-Jurado, Alejandro, Anducho-Reyes, Miguel Angel
Formato: Online
Lenguaje:spa
Publicado: Universidad de Sonora 2022
Acceso en línea:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1736
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id biotecnia-article-1736
record_format ojs
spelling biotecnia-article-17362022-10-28T15:06:34Z Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms Identificación de rizobacterias aisladas de Tagetes coronopifolia y Tagetes terniflora (Cempasúchitl) y evaluación de su capacidad inhibitoria in vitro de microorganismos fitopatógenos Castillo-Ortega, Laura Sofía Mercado-Flores, Yuridia Téllez-Jurado, Alejandro Anducho-Reyes, Miguel Angel rhizobacteria, endophytic bacteria, biological control, antagonistic plants rizobacterias, bacterias endofíticas, control biológico, plantas antagónicas Tagetes is a group of plants commonly known as cempasúchil or “flower of the dead”, which are mainly recognized for their use in the food industry, ornamental horticulture, therapeutic herbalism and culture. Tagetes coronopifolia and terniflora are two species plants of this genus, which are used in crop rotation to control phytopathogenic nematodes. In the curent work, 581 strains were isolated from samples of rhizosphere soil, soil attached to the root and the interior of the root at 30, 60, 90 and 120 days of growth, in greenhouse cultures. From the total isolated rhizobacteria strains, 22 presented antagonistic activity to Fusarium sp. and 53 to Stenocarpella maydis, both. pathogenic fungi in corn, while 37 strains showed antagonism to the pathogenic bacteria Clavibacter michiganensis subsp michiganensis. According to the percentage of inhibition of the phytopathogens, 10 rhizobacteria were selected for molecular identification using a fragment of approximately 1484 bp of the 16SrDNA gene. The similarity analysis using the NCBI BLAST tool, showed that the rhizobacteria belong to the genera Bacillus and Pseudomonas, which are used as biocontrol agents. El género Tagetes, es un grupo de plantas conocidas comúnmente como cempasúchil o flor del muerto, las cuales, son reconocidas principalmente por su uso en la industria alimentaria, horticultura ornamental, terapéutico herbolario y cultural. Tagetes coronopifolia y terniflora, son dos plantas de este género, empleadas en la rotación de cultivos para el control de nemátodos fitopatógenos. En este trabajo se realizó el aislamiento de 581 cepas a partir de muestras de suelo rizosférico, suelo adherido a la raíz y el interior de la raíz a los 30, 60, 90 y 120 días de crecimiento, en cultivos de invernadero. Del total de cepas de rizobacterias aisladas, 22 presentaron actividad antagónica a Fusarium sp. y 53 a Stenocarpella maydis, ambos. hongos patógenos de maíz, mientras que 37 cepas mostraron antagonismo a la bacteria fitopatógena Clavibacter michiganensis subsp michiganensis. De acuerdo con el porcentaje de inhibición de los patógenos en estudio, se seleccionaron 10 rizobacterias para su identificaron molecular utilizando un fragmento de aproximadamente 1484 pb del gen 16SrDNA. El análisis de similitud utilizando la herramienta BLAST del NCBI, arrojó como resultado que las rizobacterias pertenecen a los géneros Bacillus y Pseudomonas, géneros utilizados como agentes de biocontrol. Universidad de Sonora 2022-10-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf https://biotecnia.unison.mx/index.php/biotecnia/article/view/1736 10.18633/biotecnia.v24i3.1736 Biotecnia; Vol. 24 No. 3 (2022): Septiembre-Diciembre; 150 - 158 Biotecnia; Vol. 24 Núm. 3 (2022): Septiembre-Diciembre; 150 - 158 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1736/722 Derechos de autor 2022 https://creativecommons.org/licenses/by-nc-sa/4.0
institution Biotecnia
collection OJS
language spa
format Online
author Castillo-Ortega, Laura Sofía
Mercado-Flores, Yuridia
Téllez-Jurado, Alejandro
Anducho-Reyes, Miguel Angel
spellingShingle Castillo-Ortega, Laura Sofía
Mercado-Flores, Yuridia
Téllez-Jurado, Alejandro
Anducho-Reyes, Miguel Angel
Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
author_facet Castillo-Ortega, Laura Sofía
Mercado-Flores, Yuridia
Téllez-Jurado, Alejandro
Anducho-Reyes, Miguel Angel
author_sort Castillo-Ortega, Laura Sofía
title Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
title_short Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
title_full Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
title_fullStr Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
title_full_unstemmed Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
title_sort identification of rhizobacteria isolated from tagetes coronopifolia and tagetes terniflora (cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
description Tagetes is a group of plants commonly known as cempasúchil or “flower of the dead”, which are mainly recognized for their use in the food industry, ornamental horticulture, therapeutic herbalism and culture. Tagetes coronopifolia and terniflora are two species plants of this genus, which are used in crop rotation to control phytopathogenic nematodes. In the curent work, 581 strains were isolated from samples of rhizosphere soil, soil attached to the root and the interior of the root at 30, 60, 90 and 120 days of growth, in greenhouse cultures. From the total isolated rhizobacteria strains, 22 presented antagonistic activity to Fusarium sp. and 53 to Stenocarpella maydis, both. pathogenic fungi in corn, while 37 strains showed antagonism to the pathogenic bacteria Clavibacter michiganensis subsp michiganensis. According to the percentage of inhibition of the phytopathogens, 10 rhizobacteria were selected for molecular identification using a fragment of approximately 1484 bp of the 16SrDNA gene. The similarity analysis using the NCBI BLAST tool, showed that the rhizobacteria belong to the genera Bacillus and Pseudomonas, which are used as biocontrol agents.
publisher Universidad de Sonora
publishDate 2022
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1736
work_keys_str_mv AT castilloortegalaurasofia identificationofrhizobacteriaisolatedfromtagetescoronopifoliaandtagetesternifloracempasuchitlandevaluationoftheirinhibitorycapacityinvitroofphytopathogenicmicroorganisms
AT mercadofloresyuridia identificationofrhizobacteriaisolatedfromtagetescoronopifoliaandtagetesternifloracempasuchitlandevaluationoftheirinhibitorycapacityinvitroofphytopathogenicmicroorganisms
AT tellezjuradoalejandro identificationofrhizobacteriaisolatedfromtagetescoronopifoliaandtagetesternifloracempasuchitlandevaluationoftheirinhibitorycapacityinvitroofphytopathogenicmicroorganisms
AT anduchoreyesmiguelangel identificationofrhizobacteriaisolatedfromtagetescoronopifoliaandtagetesternifloracempasuchitlandevaluationoftheirinhibitorycapacityinvitroofphytopathogenicmicroorganisms
AT castilloortegalaurasofia identificacionderizobacteriasaisladasdetagetescoronopifoliaytagetesternifloracempasuchitlyevaluaciondesucapacidadinhibitoriainvitrodemicroorganismosfitopatogenos
AT mercadofloresyuridia identificacionderizobacteriasaisladasdetagetescoronopifoliaytagetesternifloracempasuchitlyevaluaciondesucapacidadinhibitoriainvitrodemicroorganismosfitopatogenos
AT tellezjuradoalejandro identificacionderizobacteriasaisladasdetagetescoronopifoliaytagetesternifloracempasuchitlyevaluaciondesucapacidadinhibitoriainvitrodemicroorganismosfitopatogenos
AT anduchoreyesmiguelangel identificacionderizobacteriasaisladasdetagetescoronopifoliaytagetesternifloracempasuchitlyevaluaciondesucapacidadinhibitoriainvitrodemicroorganismosfitopatogenos
_version_ 1756584837511118848

Publicaciones similares