Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.

Bacterial canker is one of the tomatoes (Solanum lycopersicum) diseases caused by the bacterium Clavibacter michiganensis subsp. michiganensis (Cmm). In this study, Cmm was identified by end-point PCR using the primers CMM5F and CMM6R. The efficacy of methanolic extracts of Agave striata and Fouquie...

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Asıl Yazarlar:	Ramírez Méndez, Jesús Eduardo, Hernández Castillo, Francisco Daniel, Tucuch Pérez, Marco Antonio, Camacho Aguilar, Isaac Irving, Arredondo Valdés, Roberto, Villarreal Quintanilla, José Ángel
Materyal Türü:	Online
Dil:	spa
Baskı/Yayın Bilgisi:	Universidad de Sonora 2022
Online Erişim:	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1751
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id	biotecnia-article-1751
record_format	ojs
spelling	biotecnia-article-17512023-05-10T21:14:27Z Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis. Efectividad biológica de extractos de Agave striata y Fouquieria splendens contra Clavibacter michiganensis Subp. michiganensis. Ramírez Méndez, Jesús Eduardo Hernández Castillo, Francisco Daniel Tucuch Pérez, Marco Antonio Camacho Aguilar, Isaac Irving Arredondo Valdés, Roberto Villarreal Quintanilla, José Ángel Clavibacter michiganensis Subsp. michiganensis, tomato, methanolic extracts, biological effectiveness. Clavibacter michiganensis Subsp. michiganensis, tomate, extractos metanólicos, efectividad biológica. Bacterial canker is one of the tomatoes (Solanum lycopersicum) diseases caused by the bacterium Clavibacter michiganensis subsp. michiganensis (Cmm). In this study, Cmm was identified by end-point PCR using the primers CMM5F and CMM6R. The efficacy of methanolic extracts of Agave striata and Fouquieria splendens on Cmm in vitro under greenhouse conditions was evaluated, as well as information about the composition of phytochemicals present in plants. The in vitro assay was performed using the plate microdilution technique from 3.9 mg/L to 1000 mg/L, the Ci50 and Ci90 of each extract was determined. In the greenhouse experiment, the effect of the extracts and an organic product "Biobacter" against Cmm was evaluated on incidence, severity, and morphometric parameters. The in vitro results indicated that the extracts of F. splendens and A. striata showed inhibition on the phytopathogen. The greenhouse trial showed that the incidence, severity and morphometric parameters were lower with F. splendens extract, subsequently, with Biobacter product. El cancro bacteriano es una de las enfermedades del tomate (Solanum lycopersicum), la cual es causada por la bacteria Clavibacter michiganensis subsp. michiganensis (Cmm). En el presente trabajo se realizó la identificación de Cmm mediante PCR de punto final utilizando los primers CMM5F y CMM6R. Se evaluó la efectividad de los extractos metanólicos de Agave striata y Fouquieria splendens sobre Cmm in vitro e invernadero, así también se obtuvo información acerca de la composición de fitoquímicos presentes en las plantas. El ensayo in vitro se realizó mediante la técnica de microdilución en placa desde los 3.9 mg/L a 1000 mg/L, se determinó la Ci50 y Ci90 de cada extracto . En el experimento en invernadero se evaluó el efecto de los extractos y un producto orgánico “Biobacter” contra Cmm, se calculó la incidencia, severidad y parámetros morfométricos. Los resultados in vitro indicaron que los extractos de F. splendens y A. striata presentan inhibición sobre el fitopatógeno, sin embargo, el extracto de F. splendens mostro control a menores concentraciones que con A striata. El ensayo en invernadero demostró que la incidencia, severidad y parámetros morfométricos fue menor con el extracto de F. splendens, así después con el producto Biobacter. Universidad de Sonora 2022-11-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1751 10.18633/biotecnia.v25i1.1751 Biotecnia; Vol. 25 No. 1 (2023): January - April; 34 - 42 Biotecnia; Vol. 25 Núm. 1 (2023): Enero - Abril; 34 - 42 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1751/734 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1751/824 Derechos de autor 2022 https://creativecommons.org/licenses/by-nc-sa/4.0
institution	Biotecnia
collection	OJS
language	spa
format	Online
author	Ramírez Méndez, Jesús Eduardo Hernández Castillo, Francisco Daniel Tucuch Pérez, Marco Antonio Camacho Aguilar, Isaac Irving Arredondo Valdés, Roberto Villarreal Quintanilla, José Ángel
spellingShingle	Ramírez Méndez, Jesús Eduardo Hernández Castillo, Francisco Daniel Tucuch Pérez, Marco Antonio Camacho Aguilar, Isaac Irving Arredondo Valdés, Roberto Villarreal Quintanilla, José Ángel Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
author_facet	Ramírez Méndez, Jesús Eduardo Hernández Castillo, Francisco Daniel Tucuch Pérez, Marco Antonio Camacho Aguilar, Isaac Irving Arredondo Valdés, Roberto Villarreal Quintanilla, José Ángel
author_sort	Ramírez Méndez, Jesús Eduardo
title	Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
title_short	Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
title_full	Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
title_fullStr	Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
title_full_unstemmed	Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.
title_sort	biological effectiveness of extracts of agave striata and fouquieria splendens against clavibacter michiganensis subp. michiganensis.
description	Bacterial canker is one of the tomatoes (Solanum lycopersicum) diseases caused by the bacterium Clavibacter michiganensis subsp. michiganensis (Cmm). In this study, Cmm was identified by end-point PCR using the primers CMM5F and CMM6R. The efficacy of methanolic extracts of Agave striata and Fouquieria splendens on Cmm in vitro under greenhouse conditions was evaluated, as well as information about the composition of phytochemicals present in plants. The in vitro assay was performed using the plate microdilution technique from 3.9 mg/L to 1000 mg/L, the Ci50 and Ci90 of each extract was determined. In the greenhouse experiment, the effect of the extracts and an organic product "Biobacter" against Cmm was evaluated on incidence, severity, and morphometric parameters. The in vitro results indicated that the extracts of F. splendens and A. striata showed inhibition on the phytopathogen. The greenhouse trial showed that the incidence, severity and morphometric parameters were lower with F. splendens extract, subsequently, with Biobacter product.
publisher	Universidad de Sonora
publishDate	2022
url	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1751
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Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.

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