PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever

PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever

Rocky Mountain spotted fever (RMSF) is an infectious disease confirmed through IgM and IgG determination obtained by indirect immunofluorescence, although it has limited clinical value because it requires up to three weeks to be reliable. Polymerase Chain Reaction (PCR) is an alternative for RMSF di...

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Glavni autori: García-Cortez, Cynthia Yadira, Alvarez-Hernandez, Gerardo, Bolado-Martínez, Enrique, Candia-Plata, Maria del Carmen, Martínez-Medina, Miguel Ángel
Format: Online
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Izdano: Universidad de Sonora 2022
Online pristup:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1790
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spelling biotecnia-article-17902023-05-10T21:14:27Z PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever Confiabilidad de PCR en punto final para el diagnóstico de fiebre manchada por Rickettsia rickettsii en hisopado de piel y orina de pacientes hospitalizados en Sonora: PCR en punto final para diagnóstico de fiebre manchada por R. rickettsii García-Cortez, Cynthia Yadira Alvarez-Hernandez, Gerardo Bolado-Martínez, Enrique Candia-Plata, Maria del Carmen Martínez-Medina, Miguel Ángel PCR, cutaneous swab, urine, Rickettsia rickettsii, reliability PCR, hisopado cutáneo, orina, Rickettsia rickettsii, confiabilidad Rocky Mountain spotted fever (RMSF) is an infectious disease confirmed through IgM and IgG determination obtained by indirect immunofluorescence, although it has limited clinical value because it requires up to three weeks to be reliable. Polymerase Chain Reaction (PCR) is an alternative for RMSF diagnosis in whole blood, but there is less certainty regarding its ability when urine and tissue samples are used. This study aims to estimate the reliability of PCR in skin swab and urine samples obtained from patients with suspected RMSF, comparing it with PCR results from whole blood samples. Samples from 110 suspected RMSF hospitalized patients, between September 2018 and October 2019, were analyzed. Reliability was calculated using the Kappa Coefficient (K). Rickettsia rickettsii was detected in 21 urine samples, finding substantial agreement (K = 0.607, 95% CI (0.385, 0.844)); meanwhile, 3 skin swabs were positive, but without statistical significance (K = 0.417, 95% CI ((-0.338, 1.172).Our findings support that urine PCR is a reliable technique to confirm clinical suspicion of RMSF in hospitalized patients. La determinación de anticuerpos IgM e IgG por inmunofluorescencia indirecta es la prueba estándar para confirmar la fiebre manchada por Rickettsia rickettsii (FMRR), aunque tiene limitado valor clínico pues requiere tres semanas para ser confiable. La Reacción en Cadena de la Polimerasa (PCR) es una opción para el diagnóstico en sangre total, pero hay menos certidumbre respecto a su capacidad al usar  muestras de orina y tejido. Nos proponemos estimar la confiabilidad de PCR en muestras de hisopado cutáneo y orina en pacientes sospechosos de FMRR, comparándole con resultados de PCR en muestras de sangre total. Se analizaron muestras de 110 pacientes sospechosos de FMRR atendidos en hospitales de Sonora, entre septiembre de 2018 y octubre de 2019. El cálculo de la confiabilidad se realizó mediante el Coeficiente de Kappa (K). Se detectó Rickettsia rickettsii en 21 muestras de orina, encontrando una concordancia sustancial (K = 0.607, IC 95% (0.385, 0.844)); en tanto, 3 hisopados cutáneos resultaron positivos, pero sin significancia estadística (K = 0.417, IC95% ((-0.338, 1.172). Nuestros hallazgos sustentan que la PCR en orina es una técnica confiable para confirmar la sospecha clínica de FMRR en pacientes hospitalizados. Universidad de Sonora 2022-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1790 Biotecnia; Vol. 25 No. 1 (2023): January - April; 140 - 146 Biotecnia; Vol. 25 Núm. 1 (2023): Enero - Abril; 140 - 146 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1790/741 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1790/837 Derechos de autor 2022 https://creativecommons.org/licenses/by-nc-sa/4.0
institution Biotecnia
collection OJS
language spa
format Online
author García-Cortez, Cynthia Yadira
Alvarez-Hernandez, Gerardo
Bolado-Martínez, Enrique
Candia-Plata, Maria del Carmen
Martínez-Medina, Miguel Ángel
spellingShingle García-Cortez, Cynthia Yadira
Alvarez-Hernandez, Gerardo
Bolado-Martínez, Enrique
Candia-Plata, Maria del Carmen
Martínez-Medina, Miguel Ángel
PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
author_facet García-Cortez, Cynthia Yadira
Alvarez-Hernandez, Gerardo
Bolado-Martínez, Enrique
Candia-Plata, Maria del Carmen
Martínez-Medina, Miguel Ángel
author_sort García-Cortez, Cynthia Yadira
title PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
title_short PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
title_full PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
title_fullStr PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
title_full_unstemmed PCR Reliability to Detect Rickettsia rickettsii through Cutaneous Swab and Urine Samples: PCR to confirm Rocky Mountain spotted fever
title_sort pcr reliability to detect rickettsia rickettsii through cutaneous swab and urine samples: pcr to confirm rocky mountain spotted fever
description Rocky Mountain spotted fever (RMSF) is an infectious disease confirmed through IgM and IgG determination obtained by indirect immunofluorescence, although it has limited clinical value because it requires up to three weeks to be reliable. Polymerase Chain Reaction (PCR) is an alternative for RMSF diagnosis in whole blood, but there is less certainty regarding its ability when urine and tissue samples are used. This study aims to estimate the reliability of PCR in skin swab and urine samples obtained from patients with suspected RMSF, comparing it with PCR results from whole blood samples. Samples from 110 suspected RMSF hospitalized patients, between September 2018 and October 2019, were analyzed. Reliability was calculated using the Kappa Coefficient (K). Rickettsia rickettsii was detected in 21 urine samples, finding substantial agreement (K = 0.607, 95% CI (0.385, 0.844)); meanwhile, 3 skin swabs were positive, but without statistical significance (K = 0.417, 95% CI ((-0.338, 1.172).Our findings support that urine PCR is a reliable technique to confirm clinical suspicion of RMSF in hospitalized patients.
publisher Universidad de Sonora
publishDate 2022
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1790
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