Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates

Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates

The objective of this work was to characterize Trichoderma isolates based on morphological and cultural characters, their vegetative compatibility and molecular variability. Morphological descriptions were made from microscopic observations of microcultures, according to Rifai, Gams and Bissett. The...

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Główni autorzy: Ynfante Martínez, Danay, Martínez-Coca, Benedicto, Peteira-Delgado, Belkis, Reyes-Duque, Yusimy, Gil, Katia, Simpson, June, Herrera-Estrella, Alfredo
Format: Online
Język:spa
Wydane: Universidad de Sonora 2023
Dostęp online:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890
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id biotecnia-article-1890
record_format ojs
institution Biotecnia
collection OJS
language spa
format Online
author Ynfante Martínez, Danay
Martínez-Coca, Benedicto
Peteira-Delgado, Belkis
Reyes-Duque, Yusimy
Gil, Katia
Simpson, June
Herrera-Estrella, Alfredo
spellingShingle Ynfante Martínez, Danay
Martínez-Coca, Benedicto
Peteira-Delgado, Belkis
Reyes-Duque, Yusimy
Gil, Katia
Simpson, June
Herrera-Estrella, Alfredo
Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
author_facet Ynfante Martínez, Danay
Martínez-Coca, Benedicto
Peteira-Delgado, Belkis
Reyes-Duque, Yusimy
Gil, Katia
Simpson, June
Herrera-Estrella, Alfredo
author_sort Ynfante Martínez, Danay
title Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
title_short Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
title_full Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
title_fullStr Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
title_full_unstemmed Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates
title_sort morpho-cultural characterization and genetic and molecular variability of trichoderma isolates: variability of trichoderma isolates
description The objective of this work was to characterize Trichoderma isolates based on morphological and cultural characters, their vegetative compatibility and molecular variability. Morphological descriptions were made from microscopic observations of microcultures, according to Rifai, Gams and Bissett. The vegetative compatibility relationships were macroscopically evaluated, and the type of reaction (compatible or incompatible) was determined. The genetic variability of isolates was determined by using the RAPD technique; with the results generated, a dendrogram was constructed based on Jaccard’s similarity coefficient and the analyses carried out using FreeTree software. The isolates exhibited similar morphological characteristics, however, they presented differences in the coloration of the colonies and the morphometry of fungal structures. The isolates showed vegetative compatibility with the species Trichoderma viride, Trichoderma asperellum and Trichoderma atroviride, as among them, which shows the genetic closeness between these genotypes. The eleven RAPD primers generated a total of 92 reproducible bands. Of them, 65 were polymorphic, for 70.7 % polymorphism; only OPH-19 showed 100 % polymorphism. The cluster analysis by UPGMA showed intraspecific variability, forming four groups. Specific individual bands were detected for isolates T.13, T.17, T.75 and T.78, important for designing specific primers for authentication, protection and monitoring in productive systems.
publisher Universidad de Sonora
publishDate 2023
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890
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spelling biotecnia-article-18902023-06-22T17:10:31Z Morpho-cultural characterization and genetic and molecular variability of Trichoderma isolates: Variability of Trichoderma isolates Caracterización morfo – cultural y variabilidad genética y molecular de aislamientos de Trichoderma : Variabilidad de aislamientos de Trichoderma Ynfante Martínez, Danay Martínez-Coca, Benedicto Peteira-Delgado, Belkis Reyes-Duque, Yusimy Gil, Katia Simpson, June Herrera-Estrella, Alfredo caracterización morfométrica, compatibilidad vegetativa, variabilidad genética. morphometric characterization, vegetative compatibility, genetic variability The objective of this work was to characterize Trichoderma isolates based on morphological and cultural characters, their vegetative compatibility and molecular variability. Morphological descriptions were made from microscopic observations of microcultures, according to Rifai, Gams and Bissett. The vegetative compatibility relationships were macroscopically evaluated, and the type of reaction (compatible or incompatible) was determined. The genetic variability of isolates was determined by using the RAPD technique; with the results generated, a dendrogram was constructed based on Jaccard’s similarity coefficient and the analyses carried out using FreeTree software. The isolates exhibited similar morphological characteristics, however, they presented differences in the coloration of the colonies and the morphometry of fungal structures. The isolates showed vegetative compatibility with the species Trichoderma viride, Trichoderma asperellum and Trichoderma atroviride, as among them, which shows the genetic closeness between these genotypes. The eleven RAPD primers generated a total of 92 reproducible bands. Of them, 65 were polymorphic, for 70.7 % polymorphism; only OPH-19 showed 100 % polymorphism. The cluster analysis by UPGMA showed intraspecific variability, forming four groups. Specific individual bands were detected for isolates T.13, T.17, T.75 and T.78, important for designing specific primers for authentication, protection and monitoring in productive systems. El trabajo tuvo como objetivo caracterizar aislados de Trichoderma sobre la base de caracteres morfo-culturales, compatibilidad vegetativa y variabilidad molecular. Las descripciones morfológicas se realizaron a partir de observaciones microscópicas de microcultivos, según Rifai, Gams y Bissett. Las relaciones de compatibilidad vegetativa se evaluaron macroscópicamente y se determinó el tipo de reacción (compatible e incompatible). La variabilidad genética de los aislamientos se determinó mediante el uso de la técnica RAPD; los resultados se analizaron por el método Jaccard mediante el paquete estadístico FreeTree. Los aislados presentaron características morfológicas similares, no obstante, mostraron diferencias en la coloración de las colonias y la morfometría de las estructuras fúngicas. Los aislamientos mostraron compatibilidad vegetativa con las especies Trichoderma viride, Trichoderma asperellum y Trichoderma atroviride, como entre ellos, lo que muestra la cercanía genética. Los 11 iniciadores RAPD generaron un total de 92 bandas reproducibles. De estas, 65 fueron polimórficas, para un 70,7 % de polimorfismo; solo OPH-19 mostró 100 % de polimorfismo. El análisis de agrupamiento por UPGMA mostró variabilidad intraespecífica, formándose cuatro grupos. Para T.13, T.17, T.75 y T.78 se detectaron bandas específicas, importante para el diseño de cebadores específicos, lo que posibilita su autenticación, protección y monitoreo en sistemas productivos. Universidad de Sonora 2023-05-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/html text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890 10.18633/biotecnia.v25i2.1890 Biotecnia; Vol. 25 No. 2 (2023): May - August; 194 - 203 Biotecnia; Vol. 25 Núm. 2 (2023): Mayo - Agosto; 194 - 203 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890/843 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890/844 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1890/894 Derechos de autor 2023 https://creativecommons.org/licenses/by-nc-sa/4.0

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