Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles
Exopolysaccharides (EPS) can be used as reducing agents and stabilizers improving the physical and chemical properties of metallic nanoparticles. This study aimed to synthesize and evaluate the physicochemical, structural, and functional characteristics of ZnO particles obtained from EPS produced by...
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Universidad de Sonora
2023
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| Acceso en línea: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950 |
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biotecnia-article-1950 |
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eng spa |
| format |
Online |
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Mendez Díaz, Diana Patricia Gutiérrez-Miceli, Federico Antonio Ventura Canseco, Lucía María Cristina Abud Archila, Miguel Valdéz Salas, Benjamín Morales Ruiz, María Candelaria Luján-Hidalgo, María Celina |
| spellingShingle |
Mendez Díaz, Diana Patricia Gutiérrez-Miceli, Federico Antonio Ventura Canseco, Lucía María Cristina Abud Archila, Miguel Valdéz Salas, Benjamín Morales Ruiz, María Candelaria Luján-Hidalgo, María Celina Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| author_facet |
Mendez Díaz, Diana Patricia Gutiérrez-Miceli, Federico Antonio Ventura Canseco, Lucía María Cristina Abud Archila, Miguel Valdéz Salas, Benjamín Morales Ruiz, María Candelaria Luján-Hidalgo, María Celina |
| author_sort |
Mendez Díaz, Diana Patricia |
| title |
Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| title_short |
Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| title_full |
Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| title_fullStr |
Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| title_full_unstemmed |
Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles |
| title_sort |
structural and functional characterization of zno-eps nanoparticles synthesized from exopolysaccharides produced by lactiplantibacillus fabifermentans bal-27-ittg: zno-eps nanoparticles |
| description |
Exopolysaccharides (EPS) can be used as reducing agents and stabilizers improving the physical and chemical properties of metallic nanoparticles. This study aimed to synthesize and evaluate the physicochemical, structural, and functional characteristics of ZnO particles obtained from EPS produced by Lactiplantibacillus fabifermentans BAL-27-ITTG. The structural characterization of the EPS exhibited a high molecular weight structure composed mainly of mannose and glucose possibly linked by α-1-4, α-1-3, α-1-6 according to the RMN spectrum. The increase in the concentration of EPS during the synthesis leads to an increase of particle size at micrometric scale decreasing the polydispersity index. The synthesis was confirmed with a UV-vis spectral scan, while the FT-IR spectrum showed absorption peaks at 600 cm-1 associated with the Zn-O bond. Synthetized nano and microparticles showed improved functional characteristics comparing to free EPS. The highest values of antioxidant activity (80.25 %), water holding capacity (3.06 g H2O/ g sample), emulsifying activity (69.85 %) and flocculant activity (69.07 %) were obtained from MpZnO-EPS synthetized with a concentration of 6 mg/mL of the reducing agent. Bacteriostatic activity was observed against Staphylococcus aureus and Lactiplantibacillus fabifermentans. The biocompatibility assay confirmed the obtention of non-hemolytic particles. |
| publisher |
Universidad de Sonora |
| publishDate |
2023 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950 |
| work_keys_str_mv |
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| _version_ |
1781317599889457152 |
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biotecnia-article-19502023-09-01T18:35:34Z Structural and functional characterization of ZnO-EPS nanoparticles synthesized from exopolysaccharides produced by Lactiplantibacillus fabifermentans BAL-27-ITTG: ZnO-EPS nanoparticles Caracterización estructural y funcional de nanopartículas de ZnO-EPS sintetizadas a partir de exopolisacáridos producidos por Lactiplantibacillus fabifermentans BAL-27-ITTG: Nanopartículas de ZnO-EPS Mendez Díaz, Diana Patricia Gutiérrez-Miceli, Federico Antonio Ventura Canseco, Lucía María Cristina Abud Archila, Miguel Valdéz Salas, Benjamín Morales Ruiz, María Candelaria Luján-Hidalgo, María Celina Nanoparticles microparticles ZnO exopolysaccharides Lactiplantibacillus fabifermentans Nanopartículas micropartículas ZnO Lactiplantibacillus fabifermentans Exopolisacáridos Exopolysaccharides (EPS) can be used as reducing agents and stabilizers improving the physical and chemical properties of metallic nanoparticles. This study aimed to synthesize and evaluate the physicochemical, structural, and functional characteristics of ZnO particles obtained from EPS produced by Lactiplantibacillus fabifermentans BAL-27-ITTG. The structural characterization of the EPS exhibited a high molecular weight structure composed mainly of mannose and glucose possibly linked by α-1-4, α-1-3, α-1-6 according to the RMN spectrum. The increase in the concentration of EPS during the synthesis leads to an increase of particle size at micrometric scale decreasing the polydispersity index. The synthesis was confirmed with a UV-vis spectral scan, while the FT-IR spectrum showed absorption peaks at 600 cm-1 associated with the Zn-O bond. Synthetized nano and microparticles showed improved functional characteristics comparing to free EPS. The highest values of antioxidant activity (80.25 %), water holding capacity (3.06 g H2O/ g sample), emulsifying activity (69.85 %) and flocculant activity (69.07 %) were obtained from MpZnO-EPS synthetized with a concentration of 6 mg/mL of the reducing agent. Bacteriostatic activity was observed against Staphylococcus aureus and Lactiplantibacillus fabifermentans. The biocompatibility assay confirmed the obtention of non-hemolytic particles. Los exopolisacáridos (EPS) pueden ser utilizados como agentes reductores confiriendo mejores propiedades físicas y químicas a las nanopartículas metálicas. Este estudio tuvo como objetivo sintetizar y evaluar las características fisicoquímicas, estructurales y funcionales de nanopartículas de ZnO producidas con EPS de Lactiplantibacillus fabifermentans BAL-27-ITTG. La caracterización estructural de los EPS exhibió una estructura de alto peso molecular compuesta principalmente de manosa y glucosa con enlaces α-1,4, α-1,3, α-1,6 de acuerdo al espectro de RMN. El aumento de la concentración de EPS en la síntesis provoca el incremento en el tamaño de las partículas en escala micrométrica disminuyendo el índice de polidispersidad. La síntesis de las nanopartículas se confirmó mediante un barrido espectral UV-vis y el espectro FT-IR mostró picos de absorción a 600 cm-1 asociados al enlace Zn-O. Las nano y micropartículas sintetizadas mostraron actividades funcionales mejoradas respecto al EPS libre. Los valores máximos en actividad antioxidante (80.25 %), capacidad de retención de agua (3.06 g H2O/g muestra), actividad emulsionante (69.85%) y actividad floculante (69.07 %) se obtuvieron con las MpZnO-EPS sintetizadas con 6 mg/mL del agente reductor. Se observó actividad bacteriostática contra Staphylococcus aureus y L. fabifermentans. El ensayo de biocompatibilidad confirmó la obtención de partículas no hemolíticas. Universidad de Sonora 2023-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/html image/jpeg https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950 10.18633/biotecnia.v25i3.1950 Biotecnia; Vol. 25 No. 3 (2023): September - December; 55 - 64 Biotecnia; Vol. 25 Núm. 3 (2023): Septiembre - Diciembre; 55 - 64 1665-1456 1665-1456 eng spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950/912 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950/913 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1950/914 Derechos de autor 2023 https://creativecommons.org/licenses/by-nc-sa/4.0 |
