Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts
Ten yeasts were isolated from samples obtained from the degradation of wood called "Palo podrido". The yeasts were isolated and identified from the sequencing of the ITS fragment. The phylogenetic analysis identified three strains of basidiomycetes of the genera Rhodotorula, Cryptococcus,...
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Universidad de Sonora
2023
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| Online Erişim: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966 |
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biotecnia-article-19662023-09-01T18:35:34Z Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts Aislamiento e identificación de levaduras de la degradación “Palo podrido” con actividad enzimática celulolítica, xilanolítica y lacasa: Aislamiento e identificación de levaduras Villalobos-Rocha, Juan Carlos Anducho-Reyes, Miguel Angel Mercado-Flores, Yuridia Tellez-Jurado, Alejandro Cellulase Laccase Lignocellulolytic yeasy Palo podrido Xylanase Celulasa Lacasa Levaduras lignocelulolíticas Palo podrido xilanasa Ten yeasts were isolated from samples obtained from the degradation of wood called "Palo podrido". The yeasts were isolated and identified from the sequencing of the ITS fragment. The phylogenetic analysis identified three strains of basidiomycetes of the genera Rhodotorula, Cryptococcus, and Leucosporidium and seven strains of ascomycetes of the genera Candida, Sugiyamaella, and Spencermartinsiella. Carbohydrate assimilation studies were carried out, and none of the isolated strains could assimilate starch; all strains assimilated glucose and xylose. Cryptococcus sp., Rhodotorula sp., and Leucosporidum sp. showed fermentative metabolism. Rhodotorula sp., Cryptococcus sp., Leucospororidium sp., Candida sp., and Sugiyamaella sp. showed both cellulolytic and xylanolytic activity on a plate. The hydrolytic enzymatic activity was influenced by the type and nitrogen concentration. The strains that showed cellulolytic and xylanolytic activity were dependent on the type and nitrogen concentration in the culture medium; only Rhodotorula sp. presented hydrolytic activity at three concentrations of total nitrogen (1, 2, and 3 %) and with only one type of nitrogen source. For its part, Cryptococcus sp. was the only strain that showed laccase activity in a medium supplemented with glycerol as a carbon source. Se aislaron diez levaduras de muestras obtenidas de la degradación de la madera denominada "Palo podrido". Las levaduras fueron aisladas e identificadas a partir de la secuenciación del fragmento ITS. El análisis filogenético identificó tres cepas de basidiomicetos de los géneros Rhodotorula, Cryptococcus y Leucosporidium y siete cepas de ascomicetos de los géneros Candida, Sugiyamaella y Spencermartinsiella. Se realizaron estudios de asimilación de carbohidratos y ninguna de las cepas aisladas pudo asimilar el almidón; todas las cepas asimilarion la glucosa y xilosa. Cryptococcus sp., Rhodotorula sp. y Leucosporidum sp. mostrron metabolismo fermentativo. Rhodotorula sp., Cryptococcus sp., Leucospororidium sp., Candida sp. y Sugiyamaella sp. mostraron actividad tanto celulolítica como xilanolítica en una placa. La actividad enzimática hidrolítica estuvo influenciada por el tipo y la concentración de nitrógeno. Las cepas que mostraron actividad celulolítica y xilanolítica fueron dependientes del tipo y concentración de nitrógeno en el medio de cultivo; sólo Rhodotorula sp. presentaron actividad hidrolítica a tres concentraciones de nitrógeno total (1, 2 y 3 %) y con un solo tipo de fuente de nitrógeno. Por su parte, Cryptococcus sp. fue la única cepa que mostró actividad lacasa en medio suplementado con glicerol como fuente de carbono. Universidad de Sonora 2023-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/html image/png https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966 Biotecnia; Vol. 25 No. 3 (2023): September - December; 25 - 35 Biotecnia; Vol. 25 Núm. 3 (2023): Septiembre - Diciembre; 25 - 35 1665-1456 1665-1456 eng https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966/904 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966/905 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966/906 Derechos de autor 2023 https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Biotecnia |
| collection |
OJS |
| language |
eng |
| format |
Online |
| author |
Villalobos-Rocha, Juan Carlos Anducho-Reyes, Miguel Angel Mercado-Flores, Yuridia Tellez-Jurado, Alejandro |
| spellingShingle |
Villalobos-Rocha, Juan Carlos Anducho-Reyes, Miguel Angel Mercado-Flores, Yuridia Tellez-Jurado, Alejandro Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| author_facet |
Villalobos-Rocha, Juan Carlos Anducho-Reyes, Miguel Angel Mercado-Flores, Yuridia Tellez-Jurado, Alejandro |
| author_sort |
Villalobos-Rocha, Juan Carlos |
| title |
Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| title_short |
Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| title_full |
Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| title_fullStr |
Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| title_full_unstemmed |
Isolated and identification of yeast of "Palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: Isolation and identification of yeasts |
| title_sort |
isolated and identification of yeast of "palo podrido" degradation with cellulolytic, xylanolytic, and laccase enzymatic activity: isolation and identification of yeasts |
| description |
Ten yeasts were isolated from samples obtained from the degradation of wood called "Palo podrido". The yeasts were isolated and identified from the sequencing of the ITS fragment. The phylogenetic analysis identified three strains of basidiomycetes of the genera Rhodotorula, Cryptococcus, and Leucosporidium and seven strains of ascomycetes of the genera Candida, Sugiyamaella, and Spencermartinsiella. Carbohydrate assimilation studies were carried out, and none of the isolated strains could assimilate starch; all strains assimilated glucose and xylose. Cryptococcus sp., Rhodotorula sp., and Leucosporidum sp. showed fermentative metabolism. Rhodotorula sp., Cryptococcus sp., Leucospororidium sp., Candida sp., and Sugiyamaella sp. showed both cellulolytic and xylanolytic activity on a plate. The hydrolytic enzymatic activity was influenced by the type and nitrogen concentration. The strains that showed cellulolytic and xylanolytic activity were dependent on the type and nitrogen concentration in the culture medium; only Rhodotorula sp. presented hydrolytic activity at three concentrations of total nitrogen (1, 2, and 3 %) and with only one type of nitrogen source. For its part, Cryptococcus sp. was the only strain that showed laccase activity in a medium supplemented with glycerol as a carbon source. |
| publisher |
Universidad de Sonora |
| publishDate |
2023 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1966 |
| work_keys_str_mv |
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