PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA

Light emitted by luminescent bacteria is sensitive to several toxic compounds; therefore, some luminous species have been used to evaluate the quality of water environments. In the present study we carried out the phenotypic characterization and molecular identification of a marine luminescent isola...

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Autores principales:	Delgado Gómez, Yolaine, Umaña Castro, Rodolfo, Solano González, Stefany, Iglesias Rodríguez, María V., Ortiz Guilarte, Eudalys, Álvarez Valcárcel, Carlos, Lugioyo Gallardo, Gladys M.
Formato:	Online
Lenguaje:	spa
Publicado:	Universidad de Sonora 2017
Acceso en línea:	https://biotecnia.unison.mx/index.php/biotecnia/article/view/441
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id	biotecnia-article-441
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spelling	biotecnia-article-4412022-05-31T17:28:29Z PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA Delgado Gómez, Yolaine Umaña Castro, Rodolfo Solano González, Stefany Iglesias Rodríguez, María V. Ortiz Guilarte, Eudalys Álvarez Valcárcel, Carlos Lugioyo Gallardo, Gladys M. luminescent bacteria Vibrionaceae Vibrio harveyi Mutilocus sequence analysis Light emitted by luminescent bacteria is sensitive to several toxic compounds; therefore, some luminous species have been used to evaluate the quality of water environments. In the present study we carried out the phenotypic characterization and molecular identification of a marine luminescent isolate (CBM-784) from the NW Cuban coast. The identification of the CBM-784 luminous isolate was based on phenotypic and genotypic characteristics. Phenotypically, the CBM-784 strain revealed the following characteristics: Gram negative, positive to oxidase and catalase reactions,bioluminescent, and facultative anaerobic respiration. The bacterial strain produces enzymes with gelatinase, lysine carboxylase and amylase activity. Taken together, these assays indicated that CBM-784 showed a high phenotypic similarity to the Vibrio harveyi ATCC 14126 strain. On the basis of 16S rRNA gene sequencing, CBM-784 was closely related to Vibrio harveyi and Vibrio rotiferianus (94% similarity). Sequence analysis of gyrB gene, has shown that CBM-784 shares taxonomic position with Vibrio campbellii and Vibrio harveyi isolates, with 95% of bootstrap value. In addition, sequence analysis of pyrH gene, grouped this isolate to the Vibrio harveyi cluster with a strong bootstrap support (99%). The multilocus sequence analysis and phenotypic characterization of CBM-784 indicated that this strain have a strong relation to Vibrio harveyi. Universidad de Sonora 2017-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf https://biotecnia.unison.mx/index.php/biotecnia/article/view/441 10.18633/biotecnia.v19i3.441 Biotecnia; Vol. 19 No. 3 (2017): Septiembre-Diciembre; 3-10 Biotecnia; Vol. 19 Núm. 3 (2017): Septiembre-Diciembre; 3-10 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/441/233 Derechos de autor 2017 Biotecnia
institution	Biotecnia
collection	OJS
language	spa
format	Online
author	Delgado Gómez, Yolaine Umaña Castro, Rodolfo Solano González, Stefany Iglesias Rodríguez, María V. Ortiz Guilarte, Eudalys Álvarez Valcárcel, Carlos Lugioyo Gallardo, Gladys M.
spellingShingle	Delgado Gómez, Yolaine Umaña Castro, Rodolfo Solano González, Stefany Iglesias Rodríguez, María V. Ortiz Guilarte, Eudalys Álvarez Valcárcel, Carlos Lugioyo Gallardo, Gladys M. PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
author_facet	Delgado Gómez, Yolaine Umaña Castro, Rodolfo Solano González, Stefany Iglesias Rodríguez, María V. Ortiz Guilarte, Eudalys Álvarez Valcárcel, Carlos Lugioyo Gallardo, Gladys M.
author_sort	Delgado Gómez, Yolaine
title	PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
title_short	PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
title_full	PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
title_fullStr	PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
title_full_unstemmed	PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA
title_sort	phenotypic characterization and molecular identification of a luminescent marine bacterum isolated from the nw shelf of cuba
description	Light emitted by luminescent bacteria is sensitive to several toxic compounds; therefore, some luminous species have been used to evaluate the quality of water environments. In the present study we carried out the phenotypic characterization and molecular identification of a marine luminescent isolate (CBM-784) from the NW Cuban coast. The identification of the CBM-784 luminous isolate was based on phenotypic and genotypic characteristics. Phenotypically, the CBM-784 strain revealed the following characteristics: Gram negative, positive to oxidase and catalase reactions,bioluminescent, and facultative anaerobic respiration. The bacterial strain produces enzymes with gelatinase, lysine carboxylase and amylase activity. Taken together, these assays indicated that CBM-784 showed a high phenotypic similarity to the Vibrio harveyi ATCC 14126 strain. On the basis of 16S rRNA gene sequencing, CBM-784 was closely related to Vibrio harveyi and Vibrio rotiferianus (94% similarity). Sequence analysis of gyrB gene, has shown that CBM-784 shares taxonomic position with Vibrio campbellii and Vibrio harveyi isolates, with 95% of bootstrap value. In addition, sequence analysis of pyrH gene, grouped this isolate to the Vibrio harveyi cluster with a strong bootstrap support (99%). The multilocus sequence analysis and phenotypic characterization of CBM-784 indicated that this strain have a strong relation to Vibrio harveyi.
publisher	Universidad de Sonora
publishDate	2017
url	https://biotecnia.unison.mx/index.php/biotecnia/article/view/441
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PHENOTYPIC CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF A LUMINESCENT MARINE BACTERUM ISOLATED FROM THE NW SHELF OF CUBA

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