Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae)
Vanilla planifolia Jacks. ex Andrews is an orchid of great economic importance, which faces problems due to overexploitation and threat of its original habitat. Therefore, the aim of the present investigation was to establish an in vitro micropropagation protocol evaluating the effect of different o...
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Universidad de Sonora
2021
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| Online Erişim: | https://biotecnia.unison.mx/index.php/biotecnia/article/view/805 |
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biotecnia-article-8052022-05-31T17:19:20Z Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) Efecto de extractos orgánicos naturales sobre la micropropagación in vitro de Vanilla planifolia Jacks. ex Andrews (Orchidaceae) Carranza Alvarez, Candy Trinidad-García, Karina Lizbeth Reyes-Hernández, Humberto Castillo-Pérez, Luis Jesús Fortanelli-Martínez, Javier vanilla, micropropagation, natural extracts Vainilla, micropropagación, extractos naturales Vanilla planifolia Jacks. ex Andrews is an orchid of great economic importance, which faces problems due to overexploitation and threat of its original habitat. Therefore, the aim of the present investigation was to establish an in vitro micropropagation protocol evaluating the effect of different organic extracts. The first stage of the in vitro culture was the seeds germination leading to the formation of protocorms. The protocorms were grown in MS media supplemented with natural extracts: MPL (banana), MPI (pineapple), MCO (coconut water) and control medium, to promote morphogenesis. Subsequently, to induce shoots rhizogenesis and acclimatization, auxin treatments (AIA and AIB) were used. The results showed that in vitro germination began at 60 days, and that the addition of organic extracts promoted the differentiation of the shoots, resulting the MPI treatment more efficient. Furthermore, the addition of 0.5 mg L-1 of IAA promoted vitroplants rooting and pre-acclimatization. These results suggest that organic extracts promote the vanilla morphogenesis, without synthetic growth regulators in the early stages of in vitro micropropagation. Vanilla planifolia Jacks. ex Andrews es una especie de la familia Orchidaceae que, al igual que otras orquídeas, presenta problemas para su reproducción natural; lo anterior, aunado a la deforestación de su hábitat y la presión de selección para el establecimiento de nuevos cultivos, ha causado que la vainilla se vea amenazada. Por lo anterior, el objetivo general de esta investigación fue generar un protocolo de regeneración in vitro de V. planifolia a través del uso de extractos naturales. Para ello, se cultivaron semillas estériles en medio MS sin reguladores de crecimiento vegetal para obtener protocormos como explantes. Una vez formados los protocormos, estos se sembraron en los medios de cultivo suplementados con los extractos naturales, MPL (plátano), MPI (piña), MCO (agua de coco) y en el medio control. Los resultados mostraron que el mejor tratamiento fue el MPI, en donde se observó la formación de 5.7 ± 3.5 brotes de 36.9 ± 7.3 mm de altura, y la formación de 2.2 ± 0.5 yemas por brote. Además, se logró la formación de 13.0 ± 1.1 raíces por brote con la adición de 0.5 mg L-1 de AIA, y la preaclimatación de las plantas in vitro. Universidad de Sonora 2021-01-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/805 10.18633/biotecnia.v23i1.805 Biotecnia; Vol. 23 No. 1 (2021): Enero-Abril; 5-12 Biotecnia; Vol. 23 Núm. 1 (2021): Enero-Abril; 5-12 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/805/479 https://biotecnia.unison.mx/index.php/biotecnia/article/view/805/522 Derechos de autor 2021 Biotecnia https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Biotecnia |
| collection |
OJS |
| language |
spa |
| format |
Online |
| author |
Carranza Alvarez, Candy Trinidad-García, Karina Lizbeth Reyes-Hernández, Humberto Castillo-Pérez, Luis Jesús Fortanelli-Martínez, Javier |
| spellingShingle |
Carranza Alvarez, Candy Trinidad-García, Karina Lizbeth Reyes-Hernández, Humberto Castillo-Pérez, Luis Jesús Fortanelli-Martínez, Javier Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| author_facet |
Carranza Alvarez, Candy Trinidad-García, Karina Lizbeth Reyes-Hernández, Humberto Castillo-Pérez, Luis Jesús Fortanelli-Martínez, Javier |
| author_sort |
Carranza Alvarez, Candy |
| title |
Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| title_short |
Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| title_full |
Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| title_fullStr |
Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| title_full_unstemmed |
Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae) |
| title_sort |
effect of natural extracts on the micropropagation of vanilla planifolia jacks. ex andrews (orchidaceae) |
| description |
Vanilla planifolia Jacks. ex Andrews is an orchid of great economic importance, which faces problems due to overexploitation and threat of its original habitat. Therefore, the aim of the present investigation was to establish an in vitro micropropagation protocol evaluating the effect of different organic extracts. The first stage of the in vitro culture was the seeds germination leading to the formation of protocorms. The protocorms were grown in MS media supplemented with natural extracts: MPL (banana), MPI (pineapple), MCO (coconut water) and control medium, to promote morphogenesis. Subsequently, to induce shoots rhizogenesis and acclimatization, auxin treatments (AIA and AIB) were used. The results showed that in vitro germination began at 60 days, and that the addition of organic extracts promoted the differentiation of the shoots, resulting the MPI treatment more efficient. Furthermore, the addition of 0.5 mg L-1 of IAA promoted vitroplants rooting and pre-acclimatization. These results suggest that organic extracts promote the vanilla morphogenesis, without synthetic growth regulators in the early stages of in vitro micropropagation. |
| publisher |
Universidad de Sonora |
| publishDate |
2021 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/805 |
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