Electrophoresis: fundamentals, advances and applications

Electrophoresis is a technique for separating biomolecules according to their mobility and nature (usually nucleic acids or proteins) in an electric field on a porous matrix, whose composition depends on the biomolecule to be analyzed. For the separation of nucleic acids, agarose matrices are used a...

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Autores principales:	Montalvo Navarro, Carlos Antonio, Lugo Flores, Marco Antonio
Formato:	Online
Idioma:	spa
Publicado em:	Universida de Sonora 2019
Acesso em linha:	https://epistemus.unison.mx/index.php/epistemus/article/view/96
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spelling	oai:http:--epistemus.unison.mx:article-962021-07-29T02:22:22Z Electrophoresis: fundamentals, advances and applications ELECTROFORÉSIS: FUNDAMENTOS, AVANCES Y APLICACIONES Montalvo Navarro, Carlos Antonio Lugo Flores, Marco Antonio electrophoresis DNA PAGE PFGEDGGE PFGE DGGE electroforésis ADN PAGE PFGE DGGE Electrophoresis is a technique for separating biomolecules according to their mobility and nature (usually nucleic acids or proteins) in an electric field on a porous matrix, whose composition depends on the biomolecule to be analyzed. For the separation of nucleic acids, agarose matrices are used and for the separation of proteins, polyacrylamide matrices are used. This technique represents a fundamental tool of quantitative analysis in various fields of biological sciences such as molecular, biochemical or proteomic biology. Among the different electrophoresis platforms, the most used in nucleic acid analysis are agarose gel electrophoresis, pulsed field electrophoresis (PFGE) or denaturing gradient gel electrophoresis (DGGE), and the most used for analysis of proteins are polyacrylamide gel electrophoresis with sodium duodecyl sulfate (SDS) and two-dimensional electrophoresis. This review will discuss the previously mentioned techniques and their recent applications. La electroforésis es una técnica para separación de biomoléculas según su movilidad y naturaleza (generalmente ácidos nucleicos o proteínas) en un campo eléctrico sobre una matríz porosa, cuya composición depende de la biomolécula a analizar. Para la separación de ácidos nucléicos se utilizan matrices de agarosa y para la separación de proteínas se utilizan matrices de poliacrilamida. Esta técnica representa una herramienta fundamental de análisis cuantitativos en diversos campos de ciencias biológicas como biología molecular, bioquímica o proteómica. Entre las distintas plataformas de electroforésis, las más utilizadas en análisis de ácidos nucléicos son la electroforésis en gel de agarosa, la electroforésis de campo pulsado (PFGE) o la electroforésis en gel con gradiente de desnaturalización (DGGE), y las más utilizadas para análisis de proteínas son la electroforésis en gel de poliacrilamida con duodecil sulfato de sodio (SDS) y la electroforésis bidimensional. Esta revisión discutirá las técnicas previamente mencionadas y sus recientes... Universida de Sonora 2019-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Academy Academia application/pdf https://epistemus.unison.mx/index.php/epistemus/article/view/96 10.36790/epistemus.v13i26.96 EPISTEMUS; Vol. 13 No. 26 (2019): Science does not stop; 48-54 EPISTEMUS; Vol. 13 Núm. 26 (2019): La ciencia no cesa; 48-54 2007-8196 2007-4530 spa https://epistemus.unison.mx/index.php/epistemus/article/view/96/70 https://creativecommons.org/licenses/by-nc-nd/4.0
institution	Epistemus
collection	OJS
language	spa
format	Online
author	Montalvo Navarro, Carlos Antonio Lugo Flores, Marco Antonio
spellingShingle	Montalvo Navarro, Carlos Antonio Lugo Flores, Marco Antonio Electrophoresis: fundamentals, advances and applications
author_facet	Montalvo Navarro, Carlos Antonio Lugo Flores, Marco Antonio
author_sort	Montalvo Navarro, Carlos Antonio
title	Electrophoresis: fundamentals, advances and applications
title_short	Electrophoresis: fundamentals, advances and applications
title_full	Electrophoresis: fundamentals, advances and applications
title_fullStr	Electrophoresis: fundamentals, advances and applications
title_full_unstemmed	Electrophoresis: fundamentals, advances and applications
title_sort	electrophoresis: fundamentals, advances and applications
description	Electrophoresis is a technique for separating biomolecules according to their mobility and nature (usually nucleic acids or proteins) in an electric field on a porous matrix, whose composition depends on the biomolecule to be analyzed. For the separation of nucleic acids, agarose matrices are used and for the separation of proteins, polyacrylamide matrices are used. This technique represents a fundamental tool of quantitative analysis in various fields of biological sciences such as molecular, biochemical or proteomic biology. Among the different electrophoresis platforms, the most used in nucleic acid analysis are agarose gel electrophoresis, pulsed field electrophoresis (PFGE) or denaturing gradient gel electrophoresis (DGGE), and the most used for analysis of proteins are polyacrylamide gel electrophoresis with sodium duodecyl sulfate (SDS) and two-dimensional electrophoresis. This review will discuss the previously mentioned techniques and their recent applications.
publisher	Universida de Sonora
publishDate	2019
url	https://epistemus.unison.mx/index.php/epistemus/article/view/96
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Electrophoresis: fundamentals, advances and applications

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