Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus

Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus

At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact...

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Glavni autori: Palafox Rivera, Patricia, Palafox Félix, Michel, Peralta Mendoza, Jorge Alberto
Format: Online
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Izdano: Universida de Sonora 2019
Online pristup:https://epistemus.unison.mx/index.php/epistemus/article/view/97
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spelling oai:http:--epistemus.unison.mx:article-972021-07-29T02:22:19Z Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus IDENTIFICACIÓN DEL GEN MECA POR PCR Y PCR-RT DE STAPHYLOCOCCUS AUREUS Palafox Rivera, Patricia Palafox Félix, Michel Peralta Mendoza, Jorge Alberto Staphylococcus aureus MRSA PCR PCR-RT Staphylococcus aureus SARM PCR PCR-RT At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in thiscase comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor. En la actualidad, la aplicación de técnicas más eficientes que contribuyan a la identificación de patógenos como Staphylococcus aureus de forma más rápida se ha vuelto esencial debido a que estos patógenos forman parte de la principal problemática en infecciones nosocomiales. La persistencia del problema se debe a que estos patógenos han desarrollado resistencia ante las amenazas del medio ambiente. El uso excesivo de sanitizantes y antibióticos como la meticilina son los causantes de la resistencia que en este caso proviene del gen mecA. La identificación de este gen es determinante para diferenciar a las cepas resistentes y no resistentes. Sin embargo, tomando en cuenta que los procesos tradicionales para identificación de este patógeno suelen ser muy tardados, se aplican técnicas como el PCR, por su eficacia y eficiencia. No obstante, técnicas como el PCRRT poseen mejoras que aportan cierta ventaja en comparación con su predecesor. Universida de Sonora 2019-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Academy Academia application/pdf https://epistemus.unison.mx/index.php/epistemus/article/view/97 10.36790/epistemus.v13i26.97 EPISTEMUS; Vol. 13 No. 26 (2019): Science does not stop; 55-58 EPISTEMUS; Vol. 13 Núm. 26 (2019): La ciencia no cesa; 55-58 2007-8196 2007-4530 spa https://epistemus.unison.mx/index.php/epistemus/article/view/97/71 https://creativecommons.org/licenses/by-nc-nd/4.0
institution Epistemus
collection OJS
language spa
format Online
author Palafox Rivera, Patricia
Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
spellingShingle Palafox Rivera, Patricia
Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
author_facet Palafox Rivera, Patricia
Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
author_sort Palafox Rivera, Patricia
title Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_short Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_full Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_fullStr Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_full_unstemmed Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_sort identifcation of the meca gene by pcr and pcr-rt of staphylococcus aureus
description At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in thiscase comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor.
publisher Universida de Sonora
publishDate 2019
url https://epistemus.unison.mx/index.php/epistemus/article/view/97
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AT palafoxriverapatricia identificaciondelgenmecaporpcrypcrrtdestaphylococcusaureus
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