Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus
Secondary metabolites present in plants have shown diverse biological properties, mainly the alkaloid group, which have been investigated for their fungicidal activity, capable of replacing synthetic products. The species of the Jatropha genus have been studied for their wide variety of secondary me...
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biotecnia-article-12162022-05-31T17:20:08Z Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus Actividad antifúngica de extractos metanólicos y alcaloides de Jatropha platyphylla contra Aspergillus parasiticus Leyva AcuÑa, Mario Alejandro Quintana Obregón, Eber Addí Soto Landeros, Federico Baez Parra, Karla Marina Montes Avila, Julio Angulo Escalante, Miguel Angel antifungal extract alkaloid identification Jatropha platyphylla antifúngico extracto alcaloide identificación Jatropha platyphylla. Secondary metabolites present in plants have shown diverse biological properties, mainly the alkaloid group, which have been investigated for their fungicidal activity, capable of replacing synthetic products. The species of the Jatropha genus have been studied for their wide variety of secondary metabolites with antimicrobial potential, becoming relevant in the search for new control alternatives against fungi with clinical and food importance such as Aspergillus parasiticus, considered as one of the main aflatoxin producer-species. In this work, the presence of alkaloids in Jatropha platyphylla was first identified by high-resolution thin layer chromatography (HPTLC) and the inhibitory activity of methanolic extracts and alkaloids was evaluated, at concentrations of 0.01, 0.1, 0.5, 1 and 2 mg/mL, on the radial growth of A. parasiticus. In addition, germination was determined from the IC50 of the treatments. A higher activity of the alkaloids was detected at 2 mg/mL, with 80 % inhibition at 48 h (P<0.05), with an IC50 of 2.7 mg/mL. These results show the alkaloids of the methanolic extract of J. platyphylla leaf as possible alternative treatment to the control of A. parasiticus. Los metabolitos secundarios presentes en plantas han demostrado ejercer diversas propiedades biológicas, principalmente el grupo de alcaloides, los cuales han sido investigados por su actividad fungicida, capaz de reemplazar a los productos sintéticos. Las especies del género Jatropha han sido estudiadas por su amplia variedad de metabolitos secundarios con potencial antimicrobiano, logrando ser relevantes en la búsqueda de nuevas alternativas al control de hongos de importancia clínica y alimentaria como Aspergillus parasiticus, considerado como una de las principales especies de hongos productores de aflatoxinas. En el presente trabajo se identificó por primera vez por cromatografía en capa fina de alta resolución (HPTLC) la presencia de alcaloides en Jatropha platyphylla y se evaluó la actividad inhibitoria de los extractos metanólicos y fracción enriquecida de alcaloides, a concentraciones de 0.01, 0.1, 0.5, 1 y 2 mg/mL, sobre el crecimiento radial de A. parasiticus. Además se determinó su germinación a partir de la CI50 de los tratamientos. Se observó una mayor actividad de los alcaloides a 2 mg/mL, con el 80 % de inhibición a las 48 h (P< 0,05), con una CI50 de 2.7 mg/mL. Estos resultados evidencian a los alcaloides de J. platyphylla como posibles tratamientos alternativos al control de A. parasiticus. Universidad de Sonora 2020-09-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1216 10.18633/biotecnia.v22i3.1216 Biotecnia; Vol. 22 No. 3 (2020): Septiembre-Diciembre; 100-107 Biotecnia; Vol. 22 Núm. 3 (2020): Septiembre-Diciembre; 100-107 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1216/461 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1216/514 Derechos de autor 2020 Biotecnia |
| institution |
Biotecnia |
| collection |
OJS |
| language |
spa |
| format |
Online |
| author |
Leyva AcuÑa, Mario Alejandro Quintana Obregón, Eber Addí Soto Landeros, Federico Baez Parra, Karla Marina Montes Avila, Julio Angulo Escalante, Miguel Angel |
| spellingShingle |
Leyva AcuÑa, Mario Alejandro Quintana Obregón, Eber Addí Soto Landeros, Federico Baez Parra, Karla Marina Montes Avila, Julio Angulo Escalante, Miguel Angel Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| author_facet |
Leyva AcuÑa, Mario Alejandro Quintana Obregón, Eber Addí Soto Landeros, Federico Baez Parra, Karla Marina Montes Avila, Julio Angulo Escalante, Miguel Angel |
| author_sort |
Leyva AcuÑa, Mario Alejandro |
| title |
Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| title_short |
Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| title_full |
Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| title_fullStr |
Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| title_full_unstemmed |
Antifungal activity of alkaloids from methanolic extract of Jatropha platyphylla against Aspergillus parasiticus |
| title_sort |
antifungal activity of alkaloids from methanolic extract of jatropha platyphylla against aspergillus parasiticus |
| description |
Secondary metabolites present in plants have shown diverse biological properties, mainly the alkaloid group,
which have been investigated for their fungicidal activity, capable of replacing synthetic products. The species of the Jatropha genus have been studied for their wide variety of secondary metabolites with antimicrobial potential, becoming relevant in the search for new control alternatives against fungi with clinical and food importance such as Aspergillus parasiticus, considered as one of the main aflatoxin producer-species. In this work, the presence of alkaloids in Jatropha platyphylla was first identified by high-resolution thin layer chromatography (HPTLC) and the inhibitory activity of methanolic extracts and alkaloids was evaluated, at concentrations of 0.01, 0.1, 0.5, 1 and 2 mg/mL, on the radial growth of A. parasiticus. In addition, germination was determined from the IC50 of the treatments. A higher activity of the alkaloids was detected at 2 mg/mL, with 80 % inhibition at 48 h (P<0.05), with an IC50 of 2.7 mg/mL. These results show the alkaloids of the methanolic extract of J. platyphylla leaf as possible alternative treatment to the control of A. parasiticus. |
| publisher |
Universidad de Sonora |
| publishDate |
2020 |
| url |
https://biotecnia.unison.mx/index.php/biotecnia/article/view/1216 |
| work_keys_str_mv |
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| _version_ |
1756584813150601216 |
