Molecular identification and evaluation of bacteria in the vegetative development and production of habanero pepper

The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growthpromoting bacteria. This research aimed to isolate growthpromoting bacteria, id...

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Hauptverfasser: Adame García, Jacel, Murillo Cuevas, Félix David, Velázquez Mendoza, Víctor, López Vázquez , Mario, Antonio Vázquez, Efraín, Cabrera Mireles, Héctor, Villegas Narváez, Jazmín
Format: Online
Sprache:spa
Veröffentlicht: Universidad de Sonora 2021
Online Zugang:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1480
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Zusammenfassung:The production of habanero pepper includes the use of chemical fertilization, which contaminates the soil and aquifer mantles. This environmental damage has motivated the search for sustainable alternatives such as growthpromoting bacteria. This research aimed to isolate growthpromoting bacteria, identify them molecularly, and evaluate their effects on the vegetative development and production of habanero pepper. Five bacterial strains were isolated and identified from samples from different land uses; the agroforestry system recorded on average the most significant amount (3.5x105 CFU g-1) of bacterial strains. Analysis of the 16S ribosomal DNA gene indicated that the bacteria were associated with species belonging to the Bacillus genus; however, the phylogenetic study shows that the identification at a species level is not possible; these strains stimulated better the seedlings growth (6.86, 6.70 and 6.76 cm, respectively). In the macro tunnel greenhouse evaluation, the EAV2 strain best stimulated the development of plants (38.63 cm) and produced better fruit weight (6.45 g).