Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters

Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters

Healthcare-associated bloodstream infections are the leading cause of morbidity and mortality in hospitalized patients. Vascular catheter-related infection is its main source. Gram-positive bacteria especially Staphylococcus spp., are the most prevalent etiological agents. The aim was to assess the...

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Główni autorzy: Lissarrague, Sabina, Schofs, Laureano, Bistoletti, Mariana, García Allende, Leonardo, Lallée, Andrea, Baldaccini, Beatriz, Sánchez Bruni, Sergio, Sparo, Monica
Format: Online
Język:spa
Wydane: Universidad de Sonora 2023
Dostęp online:https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858
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spelling biotecnia-article-18582023-06-22T17:10:31Z Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters Péptido antimicrobiano AP7121: inhibición de adhesión bacteriana en catéteres vasculares: Péptido antimicrobiano AP7121 en catéteres Lissarrague, Sabina Schofs, Laureano Bistoletti, Mariana García Allende, Leonardo Lallée, Andrea Baldaccini, Beatriz Sánchez Bruni, Sergio Sparo, Monica péptido antimicrobiano AP7121 catéter vascular adhesión bacteriana inhibición antimicrobial peptide AP7121 inhibition vascular catheter bacterial adherence Healthcare-associated bloodstream infections are the leading cause of morbidity and mortality in hospitalized patients. Vascular catheter-related infection is its main source. Gram-positive bacteria especially Staphylococcus spp., are the most prevalent etiological agents. The aim was to assess the inhibitory activity of the AP7121 on the Staphylococcus aureus adherence in vascular catheters. The biofilm-producer strain S. aureus ATCC 35556 (SA) was used. MIC AP7121 for SA was performed. 20 mm segments of the vascular catheter (n=3) were inoculated with 104 UFC mL-1 of SA. Three different treatment schemes (A: simultaneous, B: previous, and C: following bacterial challenge) using 1 x MIC AP7121 were tested. Control groups were included in each scheme. The MICAP7121 was 0.48 mg/L. A reduction of 2log10 representing a decrease of 99 % of viable SA cells was achieved with schemes A and B. The post-challenge treatment with AP7121 (scheme C) produced a reduction of 1log10 representing a decrease of 90 % of viable SA cells. The results observed in this work suggest a fast antimicrobial activity of AP7121 that could be beneficial to reduce bacterial adherence on medical devices such as vascular catheters Las infecciones del torrente sanguíneo asociadas a los cuidados de la salud constituyen una de las principales causas de morbilidad y mortalidad. La infección relacionada con catéteres vasculares es su fuente principal. Las bacterias Gram positivas, especialmente Staphylococcus spp., constituyen uno de los agentes etiológicos más frecuentes. Objetivo: evaluar la actividad inhibitoria del AP7121 sobre la adherencia de Staphylococcus aureus en catéteres vasculares. Se utilizó la cepa productora de biofilm S. aureus ATCC 35556 (SA). Se estimó CIMAP7121 para SA, se inocularon segmentos de 20 mm de catéter vascular (n=3) con 104 UFC mL-1 de SA. Se probaron tres esquemas diferentes (A: simultáneo, B: anterior y C: después del desafío bacteriano) utilizando 1 x CIMAP7121. Se incluyeron grupos de control en cada esquema. La CIMAP7121 fue 0,48 mg/L. Con los esquemas A y B se logró una reducción de 2 logaritmos que representa una disminución del 99 % de células viables. El tratamiento posterior a la exposición con AP7121 (esquema C) produjo una reducción de 1 logaritmo que representa una disminución del 90 % de células viables. Estos resultados sugieren una rápida actividad antimicrobiana de AP7121 que podría ser beneficiosa para reducir la adherencia bacteriana en catéteres vasculares. Universidad de Sonora 2023-05-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/html text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858 10.18633/biotecnia.v25i2.1858 Biotecnia; Vol. 25 No. 2 (2023): May - August; 165 - 168 Biotecnia; Vol. 25 Núm. 2 (2023): Mayo - Agosto; 165 - 168 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858/811 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858/812 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858/890 Derechos de autor 2023 https://creativecommons.org/licenses/by-nc-sa/4.0
institution Biotecnia
collection OJS
language spa
format Online
author Lissarrague, Sabina
Schofs, Laureano
Bistoletti, Mariana
García Allende, Leonardo
Lallée, Andrea
Baldaccini, Beatriz
Sánchez Bruni, Sergio
Sparo, Monica
spellingShingle Lissarrague, Sabina
Schofs, Laureano
Bistoletti, Mariana
García Allende, Leonardo
Lallée, Andrea
Baldaccini, Beatriz
Sánchez Bruni, Sergio
Sparo, Monica
Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
author_facet Lissarrague, Sabina
Schofs, Laureano
Bistoletti, Mariana
García Allende, Leonardo
Lallée, Andrea
Baldaccini, Beatriz
Sánchez Bruni, Sergio
Sparo, Monica
author_sort Lissarrague, Sabina
title Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
title_short Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
title_full Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
title_fullStr Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
title_full_unstemmed Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters
title_sort inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide ap7121: antimicrobial peptide ap7121 in catheters
description Healthcare-associated bloodstream infections are the leading cause of morbidity and mortality in hospitalized patients. Vascular catheter-related infection is its main source. Gram-positive bacteria especially Staphylococcus spp., are the most prevalent etiological agents. The aim was to assess the inhibitory activity of the AP7121 on the Staphylococcus aureus adherence in vascular catheters. The biofilm-producer strain S. aureus ATCC 35556 (SA) was used. MIC AP7121 for SA was performed. 20 mm segments of the vascular catheter (n=3) were inoculated with 104 UFC mL-1 of SA. Three different treatment schemes (A: simultaneous, B: previous, and C: following bacterial challenge) using 1 x MIC AP7121 were tested. Control groups were included in each scheme. The MICAP7121 was 0.48 mg/L. A reduction of 2log10 representing a decrease of 99 % of viable SA cells was achieved with schemes A and B. The post-challenge treatment with AP7121 (scheme C) produced a reduction of 1log10 representing a decrease of 90 % of viable SA cells. The results observed in this work suggest a fast antimicrobial activity of AP7121 that could be beneficial to reduce bacterial adherence on medical devices such as vascular catheters
publisher Universidad de Sonora
publishDate 2023
url https://biotecnia.unison.mx/index.php/biotecnia/article/view/1858
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