EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH

The organic extracts from Heliopsis longipes roots possess interesting biological and pharmacological activities. However, the effect on Aspergillus parasiticus has not been investigated so far. This study was aimed to evaluate the antifungal effect of the ethanolic extract from H. longipes roots ag...

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Asıl Yazarlar:	Velez-Haro, John M., Buitimea-Cantúa, Nydia E., Rosas-Burgos, Ema C., Molina-Torres, Jorge, Buitimea-Cantúa, Génesis V.
Materyal Türü:	Online
Dil:	eng
Baskı/Yayın Bilgisi:	Universidad de Sonora 2018
Online Erişim:	https://biotecnia.unison.mx/index.php/biotecnia/article/view/720
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id	biotecnia-article-720
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spelling	biotecnia-article-7202022-05-31T17:22:20Z EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH Velez-Haro, John M. Buitimea-Cantúa, Nydia E. Rosas-Burgos, Ema C. Molina-Torres, Jorge Buitimea-Cantúa, Génesis V. Aflatoxins production Alkamide content Antifungal effect Aspergillus parasiticus Heliopsis longipes roots The organic extracts from Heliopsis longipes roots possess interesting biological and pharmacological activities. However, the effect on Aspergillus parasiticus has not been investigated so far. This study was aimed to evaluate the antifungal effect of the ethanolic extract from H. longipes roots against A. parasiticus growth. Four extract concentrations, 50, 75, 150 and 200 μg/mL were evaluated for antifungal effect, and the anti-aflatoxigenic assay was tested at 150 and 200 μg/mL. The minimal inhibitory concentration that inhibited 50 and 99% of growth (MIC50 and MIC99) were determined. Ethanolic extract was characterized by GC-EIMS analysis and its main bioactive compounds were identified. All tested concentrations inhibit the radial growth of A. parasiticus and the MIC50 and MIC99 were 116.94 and 1593.98 μg/mL, respectively. These results showed the first evidence of the antifungal effect of H. longipes on the radial growth and spore germination rate of this pathogenic fungus. The antifungal activity of H. longipes extract was attributed to affinin (7.24 ± 0.13 mg/g of fresh tissue), the most abundant alkamide detected by GC-EIMS analysis. Although, the extract did not inhibit the aflatoxins´ production, it can be used to prevent A. parasiticus growth before the mycotoxins production occur. Therefore, the extract has potential as natural antifungal agent against A. parasiticus contamination. Universidad de Sonora 2018-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf https://biotecnia.unison.mx/index.php/biotecnia/article/view/720 10.18633/biotecnia.v20i3.720 Biotecnia; Vol. 20 No. 3 (2018): Septiembre-Diciembre; 127-134 Biotecnia; Vol. 20 Núm. 3 (2018): Septiembre-Diciembre; 127-134 1665-1456 1665-1456 eng https://biotecnia.unison.mx/index.php/biotecnia/article/view/720/288 Derechos de autor 2018 Biotecnia
institution	Biotecnia
collection	OJS
language	eng
format	Online
author	Velez-Haro, John M. Buitimea-Cantúa, Nydia E. Rosas-Burgos, Ema C. Molina-Torres, Jorge Buitimea-Cantúa, Génesis V.
spellingShingle	Velez-Haro, John M. Buitimea-Cantúa, Nydia E. Rosas-Burgos, Ema C. Molina-Torres, Jorge Buitimea-Cantúa, Génesis V. EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
author_facet	Velez-Haro, John M. Buitimea-Cantúa, Nydia E. Rosas-Burgos, Ema C. Molina-Torres, Jorge Buitimea-Cantúa, Génesis V.
author_sort	Velez-Haro, John M.
title	EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
title_short	EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
title_full	EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
title_fullStr	EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
title_full_unstemmed	EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH
title_sort	effect of the roots extract from heliopsis longipes on aspergillus parasiticus growth
description	The organic extracts from Heliopsis longipes roots possess interesting biological and pharmacological activities. However, the effect on Aspergillus parasiticus has not been investigated so far. This study was aimed to evaluate the antifungal effect of the ethanolic extract from H. longipes roots against A. parasiticus growth. Four extract concentrations, 50, 75, 150 and 200 μg/mL were evaluated for antifungal effect, and the anti-aflatoxigenic assay was tested at 150 and 200 μg/mL. The minimal inhibitory concentration that inhibited 50 and 99% of growth (MIC50 and MIC99) were determined. Ethanolic extract was characterized by GC-EIMS analysis and its main bioactive compounds were identified. All tested concentrations inhibit the radial growth of A. parasiticus and the MIC50 and MIC99 were 116.94 and 1593.98 μg/mL, respectively. These results showed the first evidence of the antifungal effect of H. longipes on the radial growth and spore germination rate of this pathogenic fungus. The antifungal activity of H. longipes extract was attributed to affinin (7.24 ± 0.13 mg/g of fresh tissue), the most abundant alkamide detected by GC-EIMS analysis. Although, the extract did not inhibit the aflatoxins´ production, it can be used to prevent A. parasiticus growth before the mycotoxins production occur. Therefore, the extract has potential as natural antifungal agent against A. parasiticus contamination.
publisher	Universidad de Sonora
publishDate	2018
url	https://biotecnia.unison.mx/index.php/biotecnia/article/view/720
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EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH

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