Whey protein partitioning in aqueous two-phase systems

Whey is a by-product of cheese manufacturing that has proteins that can be recovered, but they are very diluted and in a mixture with lactose, which limits their use. The aqueous two-phase extraction technique (ATPE) is a procedure with potential to separate proteins. The objective was to evaluate t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valle Guadarrama, Salvador, Domínguez Puerto, Ricardo, Guerra Ramírez, Diana
Formato:	Online
Lenguaje:	spa
Publicado:	Universidad de Sonora 2021
Acceso en línea:	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1263
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	biotecnia-article-1263
record_format	ojs
spelling	biotecnia-article-12632022-05-31T17:39:51Z Whey protein partitioning in aqueous two-phase systems Partición de proteína de lactosuero en un sistema de extracción acuosa en dos fases Valle Guadarrama, Salvador Domínguez Puerto, Ricardo Guerra Ramírez, Diana ATPE proteína/(NH4)2SO4 proteínas del suero de queso inspección de alto rendimiento separación de lactosa ATPE high throughput screening lactose separation protein/(NH4)2SO4 system whey proteins Whey is a by-product of cheese manufacturing that has proteins that can be recovered, but they are very diluted and in a mixture with lactose, which limits their use. The aqueous two-phase extraction technique (ATPE) is a procedure with potential to separate proteins. The objective was to evaluate the behavior of an aqueous protein-(NH4)2SO4 extraction system applied for the recovery of whey proteins, considering the reduction of lactose. A binodal phase diagram was constructed using a high throughput screening technique. Ammonium sulfate was dissolved at concentrations between 22 and 40 % using whey as solvent. The volume of the top phase of the systems was less than that of the bottom one, but the increase in (NH4)2SO4 caused a gradual increase at the top phase. Around 80 % of whey protein concentrated at the top phase, while over 79 % of lactose was at the bottom phase. The ATPE technique has the potential to separate proteins from whey and the best alternative is based on systems with 34 % (NH4)2SO4. El lactosuero es un subproducto de la fabricación de queso que contiene proteínas que pueden recuperarse, pero están muy diluidas y en mezcla con lactosa, lo que limita su aprovechamiento. La técnica de extracción acuosa en dos fases (ATPE, por sus siglas en inglés) es un procedimiento con potencial para separar proteínas. El objetivo fue evaluar el comportamiento de un sistema de extracción acuosa proteína-(NH4)2SO4 aplicado para la recuperación de proteínas de lactosuero, atendiendo la reducción de lactosa. Se construyó un diagrama binodal de fases mediante una técnica de inspección de alto rendimiento. Se prepararon soluciones de (NH4)2SO4 en concentraciones entre 22 y 40 % usando lactosuero como disolvente. El volumen de la fase superior de los sistemas fue menor que el de la inferior, pero el incremento de (NH4)2SO4 causó aumento gradual de la fase superior. La proteína de lactosuero se concentró en mayor cantidad en la fase superior alrededor del 80 %, en tanto que más del 79 % de la lactosa se separó en la fase inferior. La técnica de ATPE tiene potencial para separar proteínas del lactosuero y la mejor alternativa se basa en sistemas con 34 % de (NH4)2SO4. Universidad de Sonora 2021-10-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original peer-reviewed articles Artículos originales evaluados por pares application/pdf text/xml https://biotecnia.unison.mx/index.php/biotecnia/article/view/1263 10.18633/biotecnia.v23i3.1263 Biotecnia; Vol. 23 No. 3 (2021): Septiembre-Diciembre Biotecnia; Vol. 23 Núm. 3 (2021): Septiembre-Diciembre 1665-1456 1665-1456 spa https://biotecnia.unison.mx/index.php/biotecnia/article/view/1263/578 https://biotecnia.unison.mx/index.php/biotecnia/article/view/1263/598 Derechos de autor 2021 Biotecnia https://creativecommons.org/licenses/by-nc-sa/4.0
institution	Biotecnia
collection	OJS
language	spa
format	Online
author	Valle Guadarrama, Salvador Domínguez Puerto, Ricardo Guerra Ramírez, Diana
spellingShingle	Valle Guadarrama, Salvador Domínguez Puerto, Ricardo Guerra Ramírez, Diana Whey protein partitioning in aqueous two-phase systems
author_facet	Valle Guadarrama, Salvador Domínguez Puerto, Ricardo Guerra Ramírez, Diana
author_sort	Valle Guadarrama, Salvador
title	Whey protein partitioning in aqueous two-phase systems
title_short	Whey protein partitioning in aqueous two-phase systems
title_full	Whey protein partitioning in aqueous two-phase systems
title_fullStr	Whey protein partitioning in aqueous two-phase systems
title_full_unstemmed	Whey protein partitioning in aqueous two-phase systems
title_sort	whey protein partitioning in aqueous two-phase systems
description	Whey is a by-product of cheese manufacturing that has proteins that can be recovered, but they are very diluted and in a mixture with lactose, which limits their use. The aqueous two-phase extraction technique (ATPE) is a procedure with potential to separate proteins. The objective was to evaluate the behavior of an aqueous protein-(NH4)2SO4 extraction system applied for the recovery of whey proteins, considering the reduction of lactose. A binodal phase diagram was constructed using a high throughput screening technique. Ammonium sulfate was dissolved at concentrations between 22 and 40 % using whey as solvent. The volume of the top phase of the systems was less than that of the bottom one, but the increase in (NH4)2SO4 caused a gradual increase at the top phase. Around 80 % of whey protein concentrated at the top phase, while over 79 % of lactose was at the bottom phase. The ATPE technique has the potential to separate proteins from whey and the best alternative is based on systems with 34 % (NH4)2SO4.
publisher	Universidad de Sonora
publishDate	2021
url	https://biotecnia.unison.mx/index.php/biotecnia/article/view/1263
work_keys_str_mv	AT valleguadarramasalvador wheyproteinpartitioninginaqueoustwophasesystems AT dominguezpuertoricardo wheyproteinpartitioninginaqueoustwophasesystems AT guerraramirezdiana wheyproteinpartitioninginaqueoustwophasesystems AT valleguadarramasalvador particiondeproteinadelactosueroenunsistemadeextraccionacuosaendosfases AT dominguezpuertoricardo particiondeproteinadelactosueroenunsistemadeextraccionacuosaendosfases AT guerraramirezdiana particiondeproteinadelactosueroenunsistemadeextraccionacuosaendosfases
_version_	1756584817518968832

Whey protein partitioning in aqueous two-phase systems

Publicaciones similares